Table 1 Effect of various treatments on... | American Society of Hematology

Table 1

Effect of various treatments on platelet activation and fibrin formation under flow

Treatment	Fraction of adhered platelets
Treatment	Types 3 and 4	PS-exposing	Fibrin-forming
Initial (0 min)
Control	0.12 ± 0.07	0.06 ± 0.03	0
After perfusion (12 min)
Control	0.79 ± 0.04	0.89 ± 0.02	0.96 ± 0.04
PPACK	0.14 ± 0.04*	0.10 ± 0.05*	0
Cathepsin G	0.10 ± 0.03*	0.16 ± 0.09*	0.02 ± 0.01*
Annexin A5	0.08 ± 0.01*	N.d.	0.01 ± 0.01*

Treatment	Fraction of adhered platelets
Treatment	Types 3 and 4	PS-exposing	Fibrin-forming
Initial (0 min)
Control	0.12 ± 0.07	0.06 ± 0.03	0
After perfusion (12 min)
Control	0.79 ± 0.04	0.89 ± 0.02	0.96 ± 0.04
PPACK	0.14 ± 0.04*	0.10 ± 0.05*	0
Cathepsin G	0.10 ± 0.03*	0.16 ± 0.09*	0.02 ± 0.01*
Annexin A5	0.08 ± 0.01*	N.d.	0.01 ± 0.01*

Adhered platelets were superfused with recalcified plasma at 250 seconds⁻¹, as described in Figure 1. Plasma was incubated with PPACK (40μM) or unlabeled annexin A5 (20 μg/mL), as indicated. Alternatively, platelets were pretreated with cathepsin G (400nM). After 0-12 minutes of perfusion, adhered platelets were classified as types 1-4 (pseudopods, lamellipods plus core, translucent without core, and bleb-forming, respectively) from phase-contrast images. Adhered platelets were counted as PS-exposing (staining with OG488-annexin A5) and forming fibrin fibers. Values reported are means ± SE (n = 3-6 experiments).

N.d. indicates not determined.

P < .01 compared with control.

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