Table 1 Antibody staining combinations... | American Society of Hematology

Table 1

Antibody staining combinations for quantitative flow cytometry and distinct cell populations

Leukocyte subpopulation	Immunophenotype	Fluorescence label
Leukocyte subpopulation	Immunophenotype	PaB	FITC	PE	PerCP	APC	APC-Cy7
Monocytes	CD14⁺ CD45⁺		CD3	CD8	CD45	CD14
Lymphocytes	SSC^low CD45^high		CD3	CD8	CD45	CD14
B lymphocytes	CD45⁺ CD19⁺		CD3	CD16/56	CD45	CD19
NK lymphocytes	CD45⁺ CD16/56⁺
NK-T lymphocytes	CD45⁺ CD16/56⁺ CD3⁺
T lymphocytes	CD45⁺ CD3⁺ or SSC^low CD3⁺
CD4⁺ T lymphocytes	CD3⁺ CD4⁺		CD4	CD69	CD3	HLA-DR
Recently activated CD4⁺ T lymphocytes	CD3⁺ CD4⁺ CD69⁺
Remotely activated CD4⁺ T lymphocytes	CD3⁺ CD4⁺ HLA-DR⁺
Naive CD4⁺ T lymphocytes (T_N)	CD3⁺ CD4⁺ CD45RA⁺ CD27⁺		CD45RA	CD27	CD3	CD4
Central memory CD4⁺ T lymphocytes (T_CM)	CD3⁺ CD4⁺ CD45RA⁻ CD27⁺
Peripheral memory CD4⁺ T cells (T_EM)	CD3⁺ CD4⁺ CD45RA⁻ CD27⁻
Terminally differentiated effector CD4⁺ T cells (T_EMRA)	CD3⁺ CD4⁺ CD45RA⁺ CD27⁻
Proliferating CD4⁺ T lymphocytes	Ki-67⁺ CD4⁺			Ki-67		CD4
Senescent CD4⁺ lymphocytes	CD3⁺ CD4⁺ CD57⁺	CD8	CD57	CD27	CD3	CD45RA	CD4
Senescent CD4⁺ lymphocytes	CD3⁺ CD4⁺ CD28⁻	CD8	CD45RA	CD27	CD3	CD28	CD4
Regulatory T cells (“natural”)	CD3⁺ CD4^low CD25^high		CD4	CD25	CD3	CD62L
Regulatory T cells (“natural”)	CD3⁺ CD4^low FoxP3⁺	CD8	CD4	CD25	CD3	FoxP3
CD8⁺ T lymphocytes	CD3⁺ CD8⁺		CD8	CD69	CD3	HLA-DR
Recently activated CD8⁺ T lymphocytes	CD3⁺ CD8⁺ CD69⁺
Remotely activated CD8⁺ T lymphocytes	CD3⁺ CD8⁺ HLA-DR⁺
Naive CD8⁺ T lymphocytes (T_N)	CD3⁺ CD8⁺ CD45RA⁺ CD27⁺		CD45RA	CD27	CD3	CD8
Central memory CD8⁺ T lymphocytes (T_CM)	CD3⁺ CD8⁺ CD45RA⁻ CD27⁺
Peripheral memory CD8⁺ T lymphocytes (T_EM)	CD3⁺ CD8⁺ CD45RA⁻ CD27⁻
Terminally differentiated effector CD8⁺ T cells (T_EMRA)	CD3⁺ CD8⁺ CD45RA⁺ CD27⁻
Proliferating CD8⁺ T lymphocytes	Ki-67⁺ CD8⁺			Ki-67		CD8
Senescent CD8⁺ lymphocytes	CD3⁺ CD8⁺ CD57⁺	CD8	CD57	CD27	CD3	CD45RA	CD4
Senescent CD8⁺ lymphocytes	CD3⁺ CD8⁺ CD28⁻	CD8	CD45RA	CD27	CD3	CD28	CD4

Leukocyte subpopulation	Immunophenotype	Fluorescence label
Leukocyte subpopulation	Immunophenotype	PaB	FITC	PE	PerCP	APC	APC-Cy7
Monocytes	CD14⁺ CD45⁺		CD3	CD8	CD45	CD14
Lymphocytes	SSC^low CD45^high		CD3	CD8	CD45	CD14
B lymphocytes	CD45⁺ CD19⁺		CD3	CD16/56	CD45	CD19
NK lymphocytes	CD45⁺ CD16/56⁺
NK-T lymphocytes	CD45⁺ CD16/56⁺ CD3⁺
T lymphocytes	CD45⁺ CD3⁺ or SSC^low CD3⁺
CD4⁺ T lymphocytes	CD3⁺ CD4⁺		CD4	CD69	CD3	HLA-DR
Recently activated CD4⁺ T lymphocytes	CD3⁺ CD4⁺ CD69⁺
Remotely activated CD4⁺ T lymphocytes	CD3⁺ CD4⁺ HLA-DR⁺
Naive CD4⁺ T lymphocytes (T_N)	CD3⁺ CD4⁺ CD45RA⁺ CD27⁺		CD45RA	CD27	CD3	CD4
Central memory CD4⁺ T lymphocytes (T_CM)	CD3⁺ CD4⁺ CD45RA⁻ CD27⁺
Peripheral memory CD4⁺ T cells (T_EM)	CD3⁺ CD4⁺ CD45RA⁻ CD27⁻
Terminally differentiated effector CD4⁺ T cells (T_EMRA)	CD3⁺ CD4⁺ CD45RA⁺ CD27⁻
Proliferating CD4⁺ T lymphocytes	Ki-67⁺ CD4⁺			Ki-67		CD4
Senescent CD4⁺ lymphocytes	CD3⁺ CD4⁺ CD57⁺	CD8	CD57	CD27	CD3	CD45RA	CD4
Senescent CD4⁺ lymphocytes	CD3⁺ CD4⁺ CD28⁻	CD8	CD45RA	CD27	CD3	CD28	CD4
Regulatory T cells (“natural”)	CD3⁺ CD4^low CD25^high		CD4	CD25	CD3	CD62L
Regulatory T cells (“natural”)	CD3⁺ CD4^low FoxP3⁺	CD8	CD4	CD25	CD3	FoxP3
CD8⁺ T lymphocytes	CD3⁺ CD8⁺		CD8	CD69	CD3	HLA-DR
Recently activated CD8⁺ T lymphocytes	CD3⁺ CD8⁺ CD69⁺
Remotely activated CD8⁺ T lymphocytes	CD3⁺ CD8⁺ HLA-DR⁺
Naive CD8⁺ T lymphocytes (T_N)	CD3⁺ CD8⁺ CD45RA⁺ CD27⁺		CD45RA	CD27	CD3	CD8
Central memory CD8⁺ T lymphocytes (T_CM)	CD3⁺ CD8⁺ CD45RA⁻ CD27⁺
Peripheral memory CD8⁺ T lymphocytes (T_EM)	CD3⁺ CD8⁺ CD45RA⁻ CD27⁻
Terminally differentiated effector CD8⁺ T cells (T_EMRA)	CD3⁺ CD8⁺ CD45RA⁺ CD27⁻
Proliferating CD8⁺ T lymphocytes	Ki-67⁺ CD8⁺			Ki-67		CD8
Senescent CD8⁺ lymphocytes	CD3⁺ CD8⁺ CD57⁺	CD8	CD57	CD27	CD3	CD45RA	CD4
Senescent CD8⁺ lymphocytes	CD3⁺ CD8⁺ CD28⁻	CD8	CD45RA	CD27	CD3	CD28	CD4

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