MALDI-TOF analysis of peptides derived from a limited tryptic digest of Fer-phosphorylated PECAM594-711Y636,701F
| Peptide . | PECAM-1 fragment . | Potential phospho-tyrosines . | Expected monoisotopic masses . | Observed monoisotopic masses . | Sequence . | ||||
|---|---|---|---|---|---|---|---|---|---|
| UN . | Phosphorylated . | ||||||||
| No PO4 . | 1* PO4 . | 2** PO4 . | Peak 1 . | Peak 2 . | |||||
| A | 644-654 | None | 1297.6 | N/A | N/A | 1296.1 | 1296.0 | 1296.0 | NHAMKPINDNK |
| B | 680(1)-690(1) | Y686 | 1326.7 | 1406.6 | N/A | 1326.7 | 1406.5* | 1407.0* | (K)DTETVY686SEVR(K) |
| C | 644-679 | Y663 | 4023.0 | 4102.9 | N/A | 4024.9 | 4022.5 | 4103.1* | N…KEPLNSDVQY663TEVQVSSAESHKDLGK |
| D | 644-680 | Y663 | 4151.1 | 4231.0 | N/A | 4151.0 | 4150.8 | 4231.0* | N…KEPLNSDVQY663TEVQVSSAESHKDLGKK |
| E | 605-643 (F636) | None | 4408.0 | N/A | N/A | 4413.1 | 4408.1 | 4408.6 | AKQMPVEMSRPAVPLLNSNNEKMSDPNMEANSHFGHNDDVR |
| F | 644-690 | Y663, Y686 | 5330.6 | 5410.6 | 5490.5 | 5333.0 | 5411.0* | 5490.9** | N…KEPLNSDVQY663TEVQVSSAESHKDLGKKDTETVY686SEVR |
| G | 644-691 | Y663, Y686 | 5458.7 | 5538.6 | 5618.6 | 5458.6 | 5539.5* | 5618.5** | N…KEPLNSDVQY663TEVQVSSAESHKDLGKKDTETVY686SEVRK |
| Peptide . | PECAM-1 fragment . | Potential phospho-tyrosines . | Expected monoisotopic masses . | Observed monoisotopic masses . | Sequence . | ||||
|---|---|---|---|---|---|---|---|---|---|
| UN . | Phosphorylated . | ||||||||
| No PO4 . | 1* PO4 . | 2** PO4 . | Peak 1 . | Peak 2 . | |||||
| A | 644-654 | None | 1297.6 | N/A | N/A | 1296.1 | 1296.0 | 1296.0 | NHAMKPINDNK |
| B | 680(1)-690(1) | Y686 | 1326.7 | 1406.6 | N/A | 1326.7 | 1406.5* | 1407.0* | (K)DTETVY686SEVR(K) |
| C | 644-679 | Y663 | 4023.0 | 4102.9 | N/A | 4024.9 | 4022.5 | 4103.1* | N…KEPLNSDVQY663TEVQVSSAESHKDLGK |
| D | 644-680 | Y663 | 4151.1 | 4231.0 | N/A | 4151.0 | 4150.8 | 4231.0* | N…KEPLNSDVQY663TEVQVSSAESHKDLGKK |
| E | 605-643 (F636) | None | 4408.0 | N/A | N/A | 4413.1 | 4408.1 | 4408.6 | AKQMPVEMSRPAVPLLNSNNEKMSDPNMEANSHFGHNDDVR |
| F | 644-690 | Y663, Y686 | 5330.6 | 5410.6 | 5490.5 | 5333.0 | 5411.0* | 5490.9** | N…KEPLNSDVQY663TEVQVSSAESHKDLGKKDTETVY686SEVR |
| G | 644-691 | Y663, Y686 | 5458.7 | 5538.6 | 5618.6 | 5458.6 | 5539.5* | 5618.5** | N…KEPLNSDVQY663TEVQVSSAESHKDLGKKDTETVY686SEVRK |
UN indicates unphosphorylated; and N/A, not applicable.
Bold type denotes peptides containing phosphate groups, with a single asterisk (*) representing addition of a single phospate moiety and 2 asterisks (**) representing addition of 2 phosphate moieties.