Oligonucleotide primers used in this study
| Primer . | Sequence (5′ –> 3′) . | Application . |
|---|---|---|
| Pk 150R | CTGGCAGGTTATAGAGCTGC | 5′ RACE |
| Pk 47R | GTGCAGACCCGCTGCCTTG | 5′ RACE |
| Pk 2a-90R | TCTCGAACTCCTGACTTCAAG | 5′ RACE |
| Pk 2a-R-con | CAAAGTGCTGGGATTACAGGC | 5′ RACE |
| Pk ex1-F | CCGGGGCCCCGCTGTC | 3′ RACE |
| Pk 69F | CAGCGGGTCTGCACCCTG | 3′ RACE |
| Pk 110-F | TGATCTACTGGCACGTTGTGGG | 3′ RACE |
| Pk −1070F | TATTCAACGCGTACAGCCTGTGATGGGAATGAC | Luciferase |
| Pk −577F | TATTCAACGCGTTTCTCACATTGAACAAATTACCA | Luciferase |
| Pk −382F | TATTCAACGCGTCCCAGGGGTGACAGCGTC | Luciferase |
| Pk −316F | TATTCAACGCGTAGTTTTCTCATCAGTTCAATGG | Luciferase |
| Pk −240F | TATTCAACGCGTGACTGGGGACTGTCCGCA | Luciferase |
| Pk −136F | TATTCAACGCGTGACCCGCAGGGTAGGTCG | Luciferase |
| Pk +6R | TATTCAAAGCTTCTCTAGCTCCAGCGGCGG | Luciferase |
| Pk P1P2-F | TTGAATGAATTAACCGAAAGAAGTAGG | PCR-ASP |
| Pk P1m-R* | CCAAAGTGCTGGGATTACAGACG | PCR-ASP |
| Pk P2m-R* | CCAAAGTGCTGGGATTACAGACA | PCR-ASP |
| JK ASP-CF | GCATGCTGCCATAGGATCATTGC | PCR-ASP |
| JK ASP-CR | GAGCCAGGAGGTGGGTTTGCC | PCR-ASP |
| Pk i1 2648R | ATGACATAGCAAAATGCAAGCA | i1-seq |
| Pk i1 2145F | TGAATTAACCGAAAGAAGTAGG | i1-seq, PCR-RFLP |
| Pk 2a-240-R | CTCGGCTCACTGCACTCTCT | PCR-RFLP |
| Primer . | Sequence (5′ –> 3′) . | Application . |
|---|---|---|
| Pk 150R | CTGGCAGGTTATAGAGCTGC | 5′ RACE |
| Pk 47R | GTGCAGACCCGCTGCCTTG | 5′ RACE |
| Pk 2a-90R | TCTCGAACTCCTGACTTCAAG | 5′ RACE |
| Pk 2a-R-con | CAAAGTGCTGGGATTACAGGC | 5′ RACE |
| Pk ex1-F | CCGGGGCCCCGCTGTC | 3′ RACE |
| Pk 69F | CAGCGGGTCTGCACCCTG | 3′ RACE |
| Pk 110-F | TGATCTACTGGCACGTTGTGGG | 3′ RACE |
| Pk −1070F | TATTCAACGCGTACAGCCTGTGATGGGAATGAC | Luciferase |
| Pk −577F | TATTCAACGCGTTTCTCACATTGAACAAATTACCA | Luciferase |
| Pk −382F | TATTCAACGCGTCCCAGGGGTGACAGCGTC | Luciferase |
| Pk −316F | TATTCAACGCGTAGTTTTCTCATCAGTTCAATGG | Luciferase |
| Pk −240F | TATTCAACGCGTGACTGGGGACTGTCCGCA | Luciferase |
| Pk −136F | TATTCAACGCGTGACCCGCAGGGTAGGTCG | Luciferase |
| Pk +6R | TATTCAAAGCTTCTCTAGCTCCAGCGGCGG | Luciferase |
| Pk P1P2-F | TTGAATGAATTAACCGAAAGAAGTAGG | PCR-ASP |
| Pk P1m-R* | CCAAAGTGCTGGGATTACAGACG | PCR-ASP |
| Pk P2m-R* | CCAAAGTGCTGGGATTACAGACA | PCR-ASP |
| JK ASP-CF | GCATGCTGCCATAGGATCATTGC | PCR-ASP |
| JK ASP-CR | GAGCCAGGAGGTGGGTTTGCC | PCR-ASP |
| Pk i1 2648R | ATGACATAGCAAAATGCAAGCA | i1-seq |
| Pk i1 2145F | TGAATTAACCGAAAGAAGTAGG | i1-seq, PCR-RFLP |
| Pk 2a-240-R | CTCGGCTCACTGCACTCTCT | PCR-RFLP |
Mutations were introduced in the −3 position in both reverse primers to increase allele specificity.