Antibodies and the related reagents used in this study
| Antigen . | Label . | Catalog no. . | Source . | Application . |
|---|---|---|---|---|
| TRA-1-81 | PE | 09-0012 | Stemgent | FACS |
| TRA-1-60 | PE | 09-0009 | Stemgent | FACS |
| SSEA-3 | PE | 09-0044 | Stemgent | FACS |
| SSEA-4 | PE | 09-0003 | Stemgent | FACS |
| Rat IgM, κ isotype control | PE | 09-0013 | Stemgent | FACS |
| Mouse IgM, κ isotype control | PE | 09-0002 | Stemgent | FACS |
| APC anti–hTRA-1-85 | APC | FAB3195A | R&D Systems | FACS |
| CD45 | PE | 555483 | BD Biosciences | FACS |
| CD43 | PE | 560199 | BD Biosciences | FACS |
| CD34 | PE | 555822 | BD Biosciences | FACS |
| Oct3/4 | PE | 560186 | BD Biosciences | FACS |
| SOX2 | PE | 560291 | BD Biosciences | FACS |
| NANOG | PE | 560483 | BD Biosciences | FACS |
| Mouse IgG1κ isotype control | FITC | 554679 | BD Biosciences | FACS |
| Mouse IgG1κ isotype control | PE | 554680 | BD Biosciences | FACS |
| Mouse IgG1κ isotype control | APC | 554681 | BD Biosciences | FACS |
| Mouse IgG2a isotype control | PE | 551438 | BD Biosciences | FACS |
| REX1 | None | 09-0019 | Stemgent | IF |
| OCT4 | None | 09-0023 | Stemgent | IF |
| SOX2 | None | 09-0024 | Stemgent | IF |
| Nanog | None | 09-0020 | Stemgent | IF |
| Goat anti–rabbit IgG | DyLight 488 | 09-0034 | Stemgent | IF |
| Alkaline phosphatase | Biotin | BAM1448 | R&D Systems | FACS |
| Streptavidin | PE | 12-4317 | eBioscience | FACS |
| CD29 | FITC | CD2901 | Caltag | FACS |
| CD29 | PE | CD2904 | Caltag | FACS |
| CD90 | APC | 559869 | BD Biosciences | FACS |
| Mouse IgG3, κ isotype control | PE | 401308 | BioLegend | FACS |
| Antigen . | Label . | Catalog no. . | Source . | Application . |
|---|---|---|---|---|
| TRA-1-81 | PE | 09-0012 | Stemgent | FACS |
| TRA-1-60 | PE | 09-0009 | Stemgent | FACS |
| SSEA-3 | PE | 09-0044 | Stemgent | FACS |
| SSEA-4 | PE | 09-0003 | Stemgent | FACS |
| Rat IgM, κ isotype control | PE | 09-0013 | Stemgent | FACS |
| Mouse IgM, κ isotype control | PE | 09-0002 | Stemgent | FACS |
| APC anti–hTRA-1-85 | APC | FAB3195A | R&D Systems | FACS |
| CD45 | PE | 555483 | BD Biosciences | FACS |
| CD43 | PE | 560199 | BD Biosciences | FACS |
| CD34 | PE | 555822 | BD Biosciences | FACS |
| Oct3/4 | PE | 560186 | BD Biosciences | FACS |
| SOX2 | PE | 560291 | BD Biosciences | FACS |
| NANOG | PE | 560483 | BD Biosciences | FACS |
| Mouse IgG1κ isotype control | FITC | 554679 | BD Biosciences | FACS |
| Mouse IgG1κ isotype control | PE | 554680 | BD Biosciences | FACS |
| Mouse IgG1κ isotype control | APC | 554681 | BD Biosciences | FACS |
| Mouse IgG2a isotype control | PE | 551438 | BD Biosciences | FACS |
| REX1 | None | 09-0019 | Stemgent | IF |
| OCT4 | None | 09-0023 | Stemgent | IF |
| SOX2 | None | 09-0024 | Stemgent | IF |
| Nanog | None | 09-0020 | Stemgent | IF |
| Goat anti–rabbit IgG | DyLight 488 | 09-0034 | Stemgent | IF |
| Alkaline phosphatase | Biotin | BAM1448 | R&D Systems | FACS |
| Streptavidin | PE | 12-4317 | eBioscience | FACS |
| CD29 | FITC | CD2901 | Caltag | FACS |
| CD29 | PE | CD2904 | Caltag | FACS |
| CD90 | APC | 559869 | BD Biosciences | FACS |
| Mouse IgG3, κ isotype control | PE | 401308 | BioLegend | FACS |