Primers used in this study
| Gene . | Primer name . | Sequence . | Direction . | Notes . |
|---|---|---|---|---|
| POU5F1 | 60OCT4F1 | GAGGAGTCCCAGGACATCAA | F | TGS |
| IRES2-SR | AGGAACTGCTTCCTTCACGA | R | ||
| SOX2 | 60SOXR1 | TCATGTAGGTCTGCGAGCTG | R | TGS |
| 60IRES3F | TGGCTCTCCTCAAGCGTATT | F | ||
| NANOGg | 60NANOGF1 | TTCCTTCCTCCATGGATCTG | F | TGS |
| IRES2-SR | AGGAACTGCTTCCTTCACGA | R | ||
| LIN28 | 60LinR | CTGCCTCACCCTCCTTCAA | F | TGS |
| 60IRES3F | TGGCTCTCCTCAAGCGTATT | R | ||
| c-MYC | 60MYCF1 | AGAGAAGCTGGCCTCCTACC | F | TGS |
| 60IRES2R | CCCTAGGAATGCTCGTCAAG | R | ||
| KLF4 | 60IRES3F | TGGCTCTCCTCAAGCGTATT | F | TGS |
| 60KLF-R1 | TGCTCAGCACTTCCTCAAGA | R | ||
| Large-T | 60LTf1 | TTAATTTGCCCTTGGACAGG | F | TGS |
| IRES2-SR | AGGAACTGCTTCCTTCACGA | R | ||
| EBNA | 60ORI-F1 | TTTTCGCTGCTTGTCCTTTT | F | TGS |
| 60EBNAr | TTCCAACCCGAAATTTGAGA | R | ||
| ACTB | HuACTBex3F | GTGATGGTGGGCATGGGTCAGAA | F | Control |
| HuACTBex4R | AAGAGTGCCTCAGGGCAGCGGAA | R | ||
| NANOG | NANOGf | TTCCTTCCTCCATGGATCTG | F | cDNA |
| NANOGgr | ATTGTTCCAGGTCTGGTTGC | R | ||
| POU5F1 | OCT4-F2 | AGTTTGTGCCAGGGTTTTTG | F | cDNA |
| OOCT4-R2 | ACTTCACCTTCCCTCCAACC | R | ||
| SOX2 | SOXf | AGAACCCCAAGATGCACAAC | F | cDNA |
| SOXr | GCGAGTAGGACATGCTGTAGG | R | ||
| LIN28 | LINf | CGGGCATCTGTAAGTGGTTC | F | cDNA |
| LINr | GTAGGTTGGCTTTCCCTGTG | R | ||
| ZFP42 | ZFP42F | TGCTCACAGTCCAGCAGGTGTTT | F | cDNA |
| ZFP42R | TCTGGTGTCTTGTCTTTGCCCGTT | R | ||
| DNMT3B | DNMT3bf | AAGTCGAAGGTGCGTCGTGC | F | cDNA |
| DNMT3br | CCCCTCGGTCTTTGCCGTTGT | R | ||
| BCR-ABL | BCL-1 | GCACAGCCGCAACGGCAA | F | cDNA |
| ABL-1 | GAGAAGGTTTTCCTTGGAGTT | R | ||
| GDF3 | GDF3F | TTGGCTTTCAGCTTCCTGTT | F | cDNA |
| GDF3R | CTGACCGCAACACAAACATT | R | ||
| TDGF1 | TDGF1F | ATTTCTACCCGGCTGTGATG | F | cDNA |
| TDGF1R | CCAGTTACTTGGGAGGCTGA | R | ||
| GAPDH | HuGAPDHex4F | GTTTACATGTTCCAATATGATTCCAC | F | Control |
| HuGAPDHex6R | CTGATGATCTTGAGGCTGTTGTCA | R |
| Gene . | Primer name . | Sequence . | Direction . | Notes . |
|---|---|---|---|---|
| POU5F1 | 60OCT4F1 | GAGGAGTCCCAGGACATCAA | F | TGS |
| IRES2-SR | AGGAACTGCTTCCTTCACGA | R | ||
| SOX2 | 60SOXR1 | TCATGTAGGTCTGCGAGCTG | R | TGS |
| 60IRES3F | TGGCTCTCCTCAAGCGTATT | F | ||
| NANOGg | 60NANOGF1 | TTCCTTCCTCCATGGATCTG | F | TGS |
| IRES2-SR | AGGAACTGCTTCCTTCACGA | R | ||
| LIN28 | 60LinR | CTGCCTCACCCTCCTTCAA | F | TGS |
| 60IRES3F | TGGCTCTCCTCAAGCGTATT | R | ||
| c-MYC | 60MYCF1 | AGAGAAGCTGGCCTCCTACC | F | TGS |
| 60IRES2R | CCCTAGGAATGCTCGTCAAG | R | ||
| KLF4 | 60IRES3F | TGGCTCTCCTCAAGCGTATT | F | TGS |
| 60KLF-R1 | TGCTCAGCACTTCCTCAAGA | R | ||
| Large-T | 60LTf1 | TTAATTTGCCCTTGGACAGG | F | TGS |
| IRES2-SR | AGGAACTGCTTCCTTCACGA | R | ||
| EBNA | 60ORI-F1 | TTTTCGCTGCTTGTCCTTTT | F | TGS |
| 60EBNAr | TTCCAACCCGAAATTTGAGA | R | ||
| ACTB | HuACTBex3F | GTGATGGTGGGCATGGGTCAGAA | F | Control |
| HuACTBex4R | AAGAGTGCCTCAGGGCAGCGGAA | R | ||
| NANOG | NANOGf | TTCCTTCCTCCATGGATCTG | F | cDNA |
| NANOGgr | ATTGTTCCAGGTCTGGTTGC | R | ||
| POU5F1 | OCT4-F2 | AGTTTGTGCCAGGGTTTTTG | F | cDNA |
| OOCT4-R2 | ACTTCACCTTCCCTCCAACC | R | ||
| SOX2 | SOXf | AGAACCCCAAGATGCACAAC | F | cDNA |
| SOXr | GCGAGTAGGACATGCTGTAGG | R | ||
| LIN28 | LINf | CGGGCATCTGTAAGTGGTTC | F | cDNA |
| LINr | GTAGGTTGGCTTTCCCTGTG | R | ||
| ZFP42 | ZFP42F | TGCTCACAGTCCAGCAGGTGTTT | F | cDNA |
| ZFP42R | TCTGGTGTCTTGTCTTTGCCCGTT | R | ||
| DNMT3B | DNMT3bf | AAGTCGAAGGTGCGTCGTGC | F | cDNA |
| DNMT3br | CCCCTCGGTCTTTGCCGTTGT | R | ||
| BCR-ABL | BCL-1 | GCACAGCCGCAACGGCAA | F | cDNA |
| ABL-1 | GAGAAGGTTTTCCTTGGAGTT | R | ||
| GDF3 | GDF3F | TTGGCTTTCAGCTTCCTGTT | F | cDNA |
| GDF3R | CTGACCGCAACACAAACATT | R | ||
| TDGF1 | TDGF1F | ATTTCTACCCGGCTGTGATG | F | cDNA |
| TDGF1R | CCAGTTACTTGGGAGGCTGA | R | ||
| GAPDH | HuGAPDHex4F | GTTTACATGTTCCAATATGATTCCAC | F | Control |
| HuGAPDHex6R | CTGATGATCTTGAGGCTGTTGTCA | R |
TGS indicates primer pairs for transgene specific sequences; and cDNA, primer pairs in cDNA sequences that can recognize endogenous expression of the corresponding genes.