Increased frequency of BZLF1-expressing cells following expression of BLIMP1α in LCL
| Cell line . | BZLF1+ cells in vector only-transfected cells, % . | BZLF1+ cells in BLIMP1α-transfected cells, % . | HA-tag+ cells in BLIMP1α-transfected cells, % . |
|---|---|---|---|
| HK-LCL | 3.56 | 8.12 | 6.96 |
| OKU-LCL | 1.02 | 7.14 | 11.36 |
| SL1-LCL | 0.0 | 6.32 | 9.9 |
| PER213 | 3.02 | 5.68 | 2.25 |
| SAL-LCL | 1.22 | 2.55 | 4.13 |
| Akata-BL | 0.12 | 4.31 | 5.12 |
| Cell line . | BZLF1+ cells in vector only-transfected cells, % . | BZLF1+ cells in BLIMP1α-transfected cells, % . | HA-tag+ cells in BLIMP1α-transfected cells, % . |
|---|---|---|---|
| HK-LCL | 3.56 | 8.12 | 6.96 |
| OKU-LCL | 1.02 | 7.14 | 11.36 |
| SL1-LCL | 0.0 | 6.32 | 9.9 |
| PER213 | 3.02 | 5.68 | 2.25 |
| SAL-LCL | 1.22 | 2.55 | 4.13 |
| Akata-BL | 0.12 | 4.31 | 5.12 |
Immunohistochemistry was used to measure the frequency of BZLF1-expressing cells in BLIMP1α-transfected cell lines. At least 1000 cells were counted in each sample. Staining for the HA tag provided an estimate of the transfection efficiency.