X-CGD iPSC lines iXC1 and iXC9, at passage numbers indicated, were used for APC-gp91 minigene ZFN targeting to the AAVS1 locus to yield 6 and 30 resultant clones after puromycin selection, respectively. By initial PCR screen, all 6 iXC1-APC-gp91 clones and 21 of 30 iXC9-APC-gp91 clones yielded the 5′ 1033 bp product expected from targeted integration into the AAVS1 locus. Of these, 5 from iXC1 and 15 from iXC9 were selected for additional characterization by Southern blot analysis (examples of this analysis for some iXC9-APC-gp91 clones are shown in Figure 4B) to determine which clones had targeted inserts at one versus both AAVS1 alleles, and which had random insert(s). Clones having targeted integration at only one AAVS1 allele and no random integration were further tested by sequencing to determine which had NHEJ additions or deletions at the putative wild-type (WT) AAVS1 allele (Table 3). This rigorous screen left 2 iXC1-APC-gp91 clones (c1, c3) and 2 iXC9-APC-gp91 clones (c9, c21) that passed all genetic analysis criteria.