Primers used for quantitative RT-PCR
| Target . | Sequence . | Amplicon, bp . | PrimerBank ID . |
|---|---|---|---|
| B2m | Forward: TTCTGGTGCTTGTCTCACTGA Reverse: CAGTATGTTCGGCTTCCCATTC | 104 | 31981890a1 |
| Bnip3 | Forward: TCCTGGGTAGAACTGCACTTC Reverse: GCTGGGCATCCAACAGTATTT | 103 | 6753198a1 |
| Car1 | Forward: GACTGGGGATATGGAAGCGAA Reverse: TGCAGGATTATAGGAGATGCTGA | 153 | 6753266a1 |
| Car2 | Forward: TCCCACCACTGGGGATACAG Reverse: CTCTTGGACGCAGCTTTATCATA | 170 | 31981657a1 |
| Car3 | Forward: TGACAGGTCTATGCTGAGGGG Reverse: CAGCGTATTTTACTCCGTCCAC | 125 | 31982861a1 |
| Car4 | Forward: TACGTGGCCCCCTCTACTG Reverse: CTGCGGGTCATACACACTG | 115 | 6671678a1 |
| Car5a | Forward: AAGCCCTTAGCCATCCTCAG Reverse: CTGCGGGTCATACACACTG | 198 | 6671680a1 |
| Car5b | Forward: AAGTCTCTTCGAGTACATTGCCA Reverse: TAAGGCTCGGTTCCTGGTTCT | 110 | 31044449a1 |
| Car6 | Forward: TGGAGCTATTCAGGGGATGATG Reverse: CCGTCTTCACGTCGATGGG | 100 | 29612611a1 |
| Car7 | Forward: CACCACTGTTGGGGCTACG Reverse: TGATGCTGAGGGACATGCAG | 175 | 28269695a1 |
| Car9 | Forward: TGCTCCAAGTGTCTGCTCAG Reverse: CAGGTGCATCCTCTTCACTGG | 126 | 21314850a1 |
| Car13 | Forward: CTCCGACCTCTCAGTATCAAGT Reverse: ATTTGTCCTCCGTGTCGTCAA | 100 | 13374567a1 |
| Car14 | Forward: AGGTGAGATTGGAACTGCACA Reverse: GGACAAGTCTTCATGCCAGGT | 76 | 26366272a1 |
| Car15 | Forward: CCCAACCCAGTCCCCTATCAA Reverse: CTGCTGACAGGAATTTACTCGAA | 160 | 13385334a1 |
| Cox4i1 | Forward: ATTGGCAAGAGAGCCATTTCTAC Reverse: CACGCCGATCAGCGTAAGT | 100 | 6753498a1 |
| Cox4i2 | Forward: CTGCCCGGAGTCTGGTAATG Reverse: CAGTCAACGTAGGGGGTCATC | 109 | 16716379a1 |
| Etfa | Forward: GCCTCATTGCTCCGTTTTCAG Reverse: GCTACTAAGCAGGACACTTCAC | 128 | 31981826a1 |
| Glut1 | Forward: CAGTTCGGCTATAACACTGGTG Reverse: GCCCCCGACAGAGAAGATG | 156 | 22094111a1 |
| Glut3 | Forward: ATGGGGACAACGAAGGTGAC Reverse: GTCTCAGGTGCATTGATGACTC | 107 | 31543726a1 |
| Hk2 | Forward: TGATCGCCTGCTTATTCACGG Reverse: AACCGCCTAGAAATCTCCAGA | 112 | 7305143a1 |
| Ldha | Forward: TGTCTCCAGCAAAGACTACTGT Reverse: GACTGTACTTGACAATGTTGGGA | 155 | 6754524a1 |
| Mct1 | Forward: TGTTAGTCGGAGCCTTCATTTC Reverse: CACTGGTCGTTGCACTGAATA | 105 | 6677995a1 |
| Mct4 | Forward: TCACGGGTTTCTCCTACGC Reverse: GCCAAAGCGGTTCACACAC | 167 | 13507630a1 |
| Nhe1 | Forward: TCATCCACCTCGGATCTTCCC Reverse: TCCTGAGAACAGGTAGCAGTC | 71 | 8567404a1 |
| Nhe2 | Forward: TGGGGTCACAACTTCTGGAGA Reverse: ACAGTGCTTATCATCCCTGTCT | 167 | 4835911a1 |
| Nhe3 | Forward: TGAAAAGCAGGACAAGGAAATCT Reverse: TTGGCCGCCTTCTTATTCTGG | 102 | 26329247a1 |
| Pdk1 | Forward: GGACTTCGGGTCAGTGAATGC Reverse: TCCTGAGAAGATTGTCGGGGA | 122 | 27369966a1 |
| Ppia | Forward: GAGCTGTTTGCAGACAAAGTTC Reverse: CCCTGGCACATGAATCCTGG | 125 | 6679439a1 |
| Rpl13a | Forward: GGGCAGGTTCTGGTATTGGAT Reverse: GGCTCGGAAATGGTAGGGG | 216 | 31981945a1 |
| Tbp | Forward: GCTCTGGAATTGTACCGCAG Reverse: CTGGCTCATAGCTCTTGGCTC | 216 | 16741283a1 |
| Target . | Sequence . | Amplicon, bp . | PrimerBank ID . |
|---|---|---|---|
| B2m | Forward: TTCTGGTGCTTGTCTCACTGA Reverse: CAGTATGTTCGGCTTCCCATTC | 104 | 31981890a1 |
| Bnip3 | Forward: TCCTGGGTAGAACTGCACTTC Reverse: GCTGGGCATCCAACAGTATTT | 103 | 6753198a1 |
| Car1 | Forward: GACTGGGGATATGGAAGCGAA Reverse: TGCAGGATTATAGGAGATGCTGA | 153 | 6753266a1 |
| Car2 | Forward: TCCCACCACTGGGGATACAG Reverse: CTCTTGGACGCAGCTTTATCATA | 170 | 31981657a1 |
| Car3 | Forward: TGACAGGTCTATGCTGAGGGG Reverse: CAGCGTATTTTACTCCGTCCAC | 125 | 31982861a1 |
| Car4 | Forward: TACGTGGCCCCCTCTACTG Reverse: CTGCGGGTCATACACACTG | 115 | 6671678a1 |
| Car5a | Forward: AAGCCCTTAGCCATCCTCAG Reverse: CTGCGGGTCATACACACTG | 198 | 6671680a1 |
| Car5b | Forward: AAGTCTCTTCGAGTACATTGCCA Reverse: TAAGGCTCGGTTCCTGGTTCT | 110 | 31044449a1 |
| Car6 | Forward: TGGAGCTATTCAGGGGATGATG Reverse: CCGTCTTCACGTCGATGGG | 100 | 29612611a1 |
| Car7 | Forward: CACCACTGTTGGGGCTACG Reverse: TGATGCTGAGGGACATGCAG | 175 | 28269695a1 |
| Car9 | Forward: TGCTCCAAGTGTCTGCTCAG Reverse: CAGGTGCATCCTCTTCACTGG | 126 | 21314850a1 |
| Car13 | Forward: CTCCGACCTCTCAGTATCAAGT Reverse: ATTTGTCCTCCGTGTCGTCAA | 100 | 13374567a1 |
| Car14 | Forward: AGGTGAGATTGGAACTGCACA Reverse: GGACAAGTCTTCATGCCAGGT | 76 | 26366272a1 |
| Car15 | Forward: CCCAACCCAGTCCCCTATCAA Reverse: CTGCTGACAGGAATTTACTCGAA | 160 | 13385334a1 |
| Cox4i1 | Forward: ATTGGCAAGAGAGCCATTTCTAC Reverse: CACGCCGATCAGCGTAAGT | 100 | 6753498a1 |
| Cox4i2 | Forward: CTGCCCGGAGTCTGGTAATG Reverse: CAGTCAACGTAGGGGGTCATC | 109 | 16716379a1 |
| Etfa | Forward: GCCTCATTGCTCCGTTTTCAG Reverse: GCTACTAAGCAGGACACTTCAC | 128 | 31981826a1 |
| Glut1 | Forward: CAGTTCGGCTATAACACTGGTG Reverse: GCCCCCGACAGAGAAGATG | 156 | 22094111a1 |
| Glut3 | Forward: ATGGGGACAACGAAGGTGAC Reverse: GTCTCAGGTGCATTGATGACTC | 107 | 31543726a1 |
| Hk2 | Forward: TGATCGCCTGCTTATTCACGG Reverse: AACCGCCTAGAAATCTCCAGA | 112 | 7305143a1 |
| Ldha | Forward: TGTCTCCAGCAAAGACTACTGT Reverse: GACTGTACTTGACAATGTTGGGA | 155 | 6754524a1 |
| Mct1 | Forward: TGTTAGTCGGAGCCTTCATTTC Reverse: CACTGGTCGTTGCACTGAATA | 105 | 6677995a1 |
| Mct4 | Forward: TCACGGGTTTCTCCTACGC Reverse: GCCAAAGCGGTTCACACAC | 167 | 13507630a1 |
| Nhe1 | Forward: TCATCCACCTCGGATCTTCCC Reverse: TCCTGAGAACAGGTAGCAGTC | 71 | 8567404a1 |
| Nhe2 | Forward: TGGGGTCACAACTTCTGGAGA Reverse: ACAGTGCTTATCATCCCTGTCT | 167 | 4835911a1 |
| Nhe3 | Forward: TGAAAAGCAGGACAAGGAAATCT Reverse: TTGGCCGCCTTCTTATTCTGG | 102 | 26329247a1 |
| Pdk1 | Forward: GGACTTCGGGTCAGTGAATGC Reverse: TCCTGAGAAGATTGTCGGGGA | 122 | 27369966a1 |
| Ppia | Forward: GAGCTGTTTGCAGACAAAGTTC Reverse: CCCTGGCACATGAATCCTGG | 125 | 6679439a1 |
| Rpl13a | Forward: GGGCAGGTTCTGGTATTGGAT Reverse: GGCTCGGAAATGGTAGGGG | 216 | 31981945a1 |
| Tbp | Forward: GCTCTGGAATTGTACCGCAG Reverse: CTGGCTCATAGCTCTTGGCTC | 216 | 16741283a1 |