Membrane proteins detected in the supernate of activated platelets
| Identification no. . | Protein name . | Spectral count . | Accession no. . | No. of TMDs . | Type of membrane protein . | Membrane protein location . | Known to be shed? . |
|---|---|---|---|---|---|---|---|
| 1 | Platelet glycoprotein V (GPV) | 1897 | P40197 | 1 | 1 | Plasma membrane | Yes23 |
| 2 | Amyloid βA4 | 1202 | P05067 | 1 | 1 | Plasma membrane | Yes24 |
| 3 | Platelet glycoprotein Ibα chain (GPIbα) | 701 | P07359 | 1 | 1 | Plasma membrane | Yes10 |
| 4 | Trem-like transcript 1 (TLT-1) | 331 | Q86YW5 | 1 | 1 | Plasma membrane | Yes*25 |
| 5 | P-selectin | 301 | P16109 | 1 | 1 | Plasma membrane, platelet α-granules | Yes26 |
| 6 | Amyloid-like protein 2 | 175 | Q06481 | 1 | 1 | Plasma membrane, nucleus | No |
| 7 | Plexin domain-containing protein 2 (Plxdc 2) | 169 | Q6UX71 | 1 | 1 | Plasma membrane | No |
| 8 | Platelet glycoprotein VI (GPVI) | 154 | Q9HCN6 | 1 | 1 | Plasma membrane | Yes11 |
| 9 | Semaphorin 4D | 145 | Q92854 | 1 | 1 | Plasma membrane | Yes15 |
| 10 | Integrin αIIb | 117 | P08514 | 1 | 1 | Plasma membrane | No |
| 11 | Fibrocystin-L | 114 | Q86WI1 | 1 | 1 | Plasma membrane | No |
| 12 | Chloride intracellular channel protein 1 (CLIC1) | 112 | O00299 | 1 | 2 | Plasma membrane, nucleus | No |
| 13 | Dystroglycan | 103 | Q14118 | 1 | 1 | Plasma membrane | No |
| 14 | Integrin β3 | 96 | P05106 | 1 | 1 | Plasma membrane | No |
| 15 | Endothelial cell selective adhesion molecule (ESAM) | 71 | Q96AP7 | 1 | 1 | Plasma membrane | No |
| 16 | SLAM family member 5 (CD84) | 67 | Q9UIB8 | 1 | 1 | Plasma membrane | No |
| 17 | CD109 | 65 | Q6YHK3 | 0 | GPI | Plasma membrane | No |
| 18 | Junctional adhesion molecule (JAM-A) | 54 | Q9Y5B2 | 1 | 1 | Plasma membrane | Yes†16 |
| 19 | Syndecan 4 | 54 | P31431 | 1 | 1 | Plasma membrane, Golgi | Yes27 |
| 20 | Intercellular adhesion molecule 2 (ICAM2) | 51 | P13598 | 1 | 1 | Plasma membrane | Yes‡28 |
| 21 | Ephrin B1 | 30 | P98172 | 1 | 1 | Plasma membrane | No |
| 22 | Lymphocyte antigen 6 complex locus protein G6f precursor | 29 | Q5SQ64 | 1 | 1 | Plasma membrane | No |
| 23 | Lysosome-associated membrane glycoprotein 2 (LAMP2) | 23 | P13473 | 1 | 1 | Plasma membrane, endosome, lysosome | No |
| 24 | Lysosomal acid phosphatase | 23 | P11117 | 1 | 1 | Plasma membrane, lysosome | No |
| 25 | Chloride intracellular channel protein 4 (CLIC4) | 22 | Q9Y696 | 1 | 2 | Plasma membrane, mitochondria, nucleus | No |
| 26 | CD40-L | 21 | P29965 | 1 | 2 | Plasma membrane | Yes29 |
| 27 | C-type lectin domain family 1 member B (CLEC1B; CLEC2) | 21 | Q9P126 | 1 | 2 | Plasma membrane | No |
| 28 | ADAM10 | 20 | O14672 | 1 | 1 | Plasma membrane | No |
| 29 | Pro-epidermal growth factor receptor (EGF) | 20 | P01133 | 1 | 1 | Plasma membrane | No |
| 30 | CD226 | 19 | Q15762 | 1 | 1 | Plasma membrane | No |
| 31 | Lysosome-associated membrane glycoprotein 1 (LAMP1) | 19 | P11279 | 1 | 1 | Plasma membrane, endosome, lysosome | No |
| 32 | Trem-like transcript 2 (TLT-2) | 15 | Q5T2D2 | 1 | 1 | Plasma membrane | No |
| 33 | Soluble calcium activated nucleotidase (SCAN-1) | 14 | Q8WVQ1 | 1 | 2 | Plasma membrane, ER, Golgi | No |
| 34 | Poliovirus receptor-related protein1 precursor | 14 | Q15223 | 1 | 1 | Plasma membrane | No |
| 35 | CD9 | 12 | P21926 | 4 | Multipass | Plasma membrane, platelet α-granule | No |
| 36 | Ectonucleotide pyrophosphatase | 12 | Q9Y6X5 | 1 | 1 | Plasma membrane | No |
| 37 | D-glucuronyl C5 epimerase | 11 | O94923 | 1 | 2 | Plasma membrane | No |
| 38 | CD166 | 10 | Q13740 | 1 | 1 | Plasma membrane | No |
| 39 | Receptor-type tyrosine phosphatase G | 10 | P23470 | 1 | 1 | Plasma membrane | No |
| 40 | Plexin B3 | 9 | Q9ULL4 | 1 | 1 | Plasma membrane | No |
| 41 | Semaphorin 7A | 9 | O75326 | 0 | GPI | Plasma membrane | No |
| 42 | Platelet endothelial cell adhesion molecule (PECAM) | 8 | P16284 | 1 | 1 | Plasma membrane, platelet α-granule | Yes30 |
| 43 | Desmoglein-1 | 8 | Q02413 | 1 | 1 | Plasma membrane | No |
| 44 | Chloride intracellular channel protein 5 | 7 | Q9NZA1 | 1 | 2 | Plasma membrane | No |
| 45 | Inositol monophosphatase 3 | 7 | Q9NX62 | 1 | 2 | Plasma membrane | No |
| 46 | Retinoic acid receptor responder protein 1 | 7 | P49788 | 1 | 2 | Plasma membrane | No |
| 47 | Receptor-type tyrosine phosphatase F | 5 | P10586 | 1 | 1 | Plasma membrane | No |
| 48 | Major prion protein | 5 | P04156 | 0 | GPI | Plasma membrane | No |
| 49 | Vesicle-associated membrane protein-associated protein A | 5 | Q9P0L0 | 1 | 4 | Plasma membrane, intracellular vesicles | No |
| 50 | Receptor-type tyrosine phosphatase J (CD148) | 4 | Q12913 | 1 | 1 | Plasma membrane | No |
| 51 | Receptor-type tyrosine phosphatase K | 4 | Q15262 | 1 | 1 | Plasma membrane | No |
| 52 | VCAM1 | 4 | P19320 | 1 | 1 | Plasma membrane | Yes§31 |
| 53 | Nicastrin | 4 | Q92542 | 1 | 1 | Plasma membrane | No |
| 54 | Extended synaptogamin-1 | 4 | Q9BSJ8 | 2 | Multipass | Plasma membrane | No |
| 55 | E-cadherin | 3 | P12830 | 1 | 1 | Plasma membrane | No |
| 56 | Endothelial cell scavenger receptor | 3 | Q14162 | 1 | 1 | Plasma membrane | No |
| 57 | IL6 receptor | 3 | P40189 | 1 | 1 | Plasma membrane | No |
| 58 | Protocadherin | 3 | Q9HC56 | 1 | 1 | Plasma membrane | No |
| 59 | Semaphorin 4B | 3 | Q9NPR2 | 1 | 1 | Plasma membrane | No |
| 60 | Sodium channel subunit β4 | 3 | Q8IWT1 | 1 | 1 | Plasma membrane | No |
| 61 | Tetraspanin 9 | 3 | O75954 | 4 | Multipass | Plasma membrane | No |
| 62 | Intestinal alkaline phosphatase | 3 | P09923 | 0 | GPI | Plasma membrane | No |
| 63 | Stabilin-1 | 3 | Q9NY15 | 1 | 1 | Plasma membrane | No |
| 64 | Kunitz-type protease inhibitor 2 | 2 | O43291 | 1 | 1 | Plasma membrane | No |
| 65 | Peptidyl-glycine α-amidating monooxygenase | 2 | P19021 | 1 | 1 | Plasma membrane | No |
| 66 | Platelet glycoprotein IX (GPIX) | 2 | P14770 | 1 | 1 | Plasma membrane | No |
| 67 | Ryanodine receptor 2 | 2 | Q92736 | 12 | Multipass | Plasma membrane | No |
| 68 | Stimulated by retinoic acid gene 6 (STRA6) protein homolog | 2 | Q9BX79 | 9 | Multipass | Plasma membrane | No |
| 69 | Transmembrane protein 56 | 2 | Q96MV1 | 6 | Multipass | Plasma membrane | No |
| Identification no. . | Protein name . | Spectral count . | Accession no. . | No. of TMDs . | Type of membrane protein . | Membrane protein location . | Known to be shed? . |
|---|---|---|---|---|---|---|---|
| 1 | Platelet glycoprotein V (GPV) | 1897 | P40197 | 1 | 1 | Plasma membrane | Yes23 |
| 2 | Amyloid βA4 | 1202 | P05067 | 1 | 1 | Plasma membrane | Yes24 |
| 3 | Platelet glycoprotein Ibα chain (GPIbα) | 701 | P07359 | 1 | 1 | Plasma membrane | Yes10 |
| 4 | Trem-like transcript 1 (TLT-1) | 331 | Q86YW5 | 1 | 1 | Plasma membrane | Yes*25 |
| 5 | P-selectin | 301 | P16109 | 1 | 1 | Plasma membrane, platelet α-granules | Yes26 |
| 6 | Amyloid-like protein 2 | 175 | Q06481 | 1 | 1 | Plasma membrane, nucleus | No |
| 7 | Plexin domain-containing protein 2 (Plxdc 2) | 169 | Q6UX71 | 1 | 1 | Plasma membrane | No |
| 8 | Platelet glycoprotein VI (GPVI) | 154 | Q9HCN6 | 1 | 1 | Plasma membrane | Yes11 |
| 9 | Semaphorin 4D | 145 | Q92854 | 1 | 1 | Plasma membrane | Yes15 |
| 10 | Integrin αIIb | 117 | P08514 | 1 | 1 | Plasma membrane | No |
| 11 | Fibrocystin-L | 114 | Q86WI1 | 1 | 1 | Plasma membrane | No |
| 12 | Chloride intracellular channel protein 1 (CLIC1) | 112 | O00299 | 1 | 2 | Plasma membrane, nucleus | No |
| 13 | Dystroglycan | 103 | Q14118 | 1 | 1 | Plasma membrane | No |
| 14 | Integrin β3 | 96 | P05106 | 1 | 1 | Plasma membrane | No |
| 15 | Endothelial cell selective adhesion molecule (ESAM) | 71 | Q96AP7 | 1 | 1 | Plasma membrane | No |
| 16 | SLAM family member 5 (CD84) | 67 | Q9UIB8 | 1 | 1 | Plasma membrane | No |
| 17 | CD109 | 65 | Q6YHK3 | 0 | GPI | Plasma membrane | No |
| 18 | Junctional adhesion molecule (JAM-A) | 54 | Q9Y5B2 | 1 | 1 | Plasma membrane | Yes†16 |
| 19 | Syndecan 4 | 54 | P31431 | 1 | 1 | Plasma membrane, Golgi | Yes27 |
| 20 | Intercellular adhesion molecule 2 (ICAM2) | 51 | P13598 | 1 | 1 | Plasma membrane | Yes‡28 |
| 21 | Ephrin B1 | 30 | P98172 | 1 | 1 | Plasma membrane | No |
| 22 | Lymphocyte antigen 6 complex locus protein G6f precursor | 29 | Q5SQ64 | 1 | 1 | Plasma membrane | No |
| 23 | Lysosome-associated membrane glycoprotein 2 (LAMP2) | 23 | P13473 | 1 | 1 | Plasma membrane, endosome, lysosome | No |
| 24 | Lysosomal acid phosphatase | 23 | P11117 | 1 | 1 | Plasma membrane, lysosome | No |
| 25 | Chloride intracellular channel protein 4 (CLIC4) | 22 | Q9Y696 | 1 | 2 | Plasma membrane, mitochondria, nucleus | No |
| 26 | CD40-L | 21 | P29965 | 1 | 2 | Plasma membrane | Yes29 |
| 27 | C-type lectin domain family 1 member B (CLEC1B; CLEC2) | 21 | Q9P126 | 1 | 2 | Plasma membrane | No |
| 28 | ADAM10 | 20 | O14672 | 1 | 1 | Plasma membrane | No |
| 29 | Pro-epidermal growth factor receptor (EGF) | 20 | P01133 | 1 | 1 | Plasma membrane | No |
| 30 | CD226 | 19 | Q15762 | 1 | 1 | Plasma membrane | No |
| 31 | Lysosome-associated membrane glycoprotein 1 (LAMP1) | 19 | P11279 | 1 | 1 | Plasma membrane, endosome, lysosome | No |
| 32 | Trem-like transcript 2 (TLT-2) | 15 | Q5T2D2 | 1 | 1 | Plasma membrane | No |
| 33 | Soluble calcium activated nucleotidase (SCAN-1) | 14 | Q8WVQ1 | 1 | 2 | Plasma membrane, ER, Golgi | No |
| 34 | Poliovirus receptor-related protein1 precursor | 14 | Q15223 | 1 | 1 | Plasma membrane | No |
| 35 | CD9 | 12 | P21926 | 4 | Multipass | Plasma membrane, platelet α-granule | No |
| 36 | Ectonucleotide pyrophosphatase | 12 | Q9Y6X5 | 1 | 1 | Plasma membrane | No |
| 37 | D-glucuronyl C5 epimerase | 11 | O94923 | 1 | 2 | Plasma membrane | No |
| 38 | CD166 | 10 | Q13740 | 1 | 1 | Plasma membrane | No |
| 39 | Receptor-type tyrosine phosphatase G | 10 | P23470 | 1 | 1 | Plasma membrane | No |
| 40 | Plexin B3 | 9 | Q9ULL4 | 1 | 1 | Plasma membrane | No |
| 41 | Semaphorin 7A | 9 | O75326 | 0 | GPI | Plasma membrane | No |
| 42 | Platelet endothelial cell adhesion molecule (PECAM) | 8 | P16284 | 1 | 1 | Plasma membrane, platelet α-granule | Yes30 |
| 43 | Desmoglein-1 | 8 | Q02413 | 1 | 1 | Plasma membrane | No |
| 44 | Chloride intracellular channel protein 5 | 7 | Q9NZA1 | 1 | 2 | Plasma membrane | No |
| 45 | Inositol monophosphatase 3 | 7 | Q9NX62 | 1 | 2 | Plasma membrane | No |
| 46 | Retinoic acid receptor responder protein 1 | 7 | P49788 | 1 | 2 | Plasma membrane | No |
| 47 | Receptor-type tyrosine phosphatase F | 5 | P10586 | 1 | 1 | Plasma membrane | No |
| 48 | Major prion protein | 5 | P04156 | 0 | GPI | Plasma membrane | No |
| 49 | Vesicle-associated membrane protein-associated protein A | 5 | Q9P0L0 | 1 | 4 | Plasma membrane, intracellular vesicles | No |
| 50 | Receptor-type tyrosine phosphatase J (CD148) | 4 | Q12913 | 1 | 1 | Plasma membrane | No |
| 51 | Receptor-type tyrosine phosphatase K | 4 | Q15262 | 1 | 1 | Plasma membrane | No |
| 52 | VCAM1 | 4 | P19320 | 1 | 1 | Plasma membrane | Yes§31 |
| 53 | Nicastrin | 4 | Q92542 | 1 | 1 | Plasma membrane | No |
| 54 | Extended synaptogamin-1 | 4 | Q9BSJ8 | 2 | Multipass | Plasma membrane | No |
| 55 | E-cadherin | 3 | P12830 | 1 | 1 | Plasma membrane | No |
| 56 | Endothelial cell scavenger receptor | 3 | Q14162 | 1 | 1 | Plasma membrane | No |
| 57 | IL6 receptor | 3 | P40189 | 1 | 1 | Plasma membrane | No |
| 58 | Protocadherin | 3 | Q9HC56 | 1 | 1 | Plasma membrane | No |
| 59 | Semaphorin 4B | 3 | Q9NPR2 | 1 | 1 | Plasma membrane | No |
| 60 | Sodium channel subunit β4 | 3 | Q8IWT1 | 1 | 1 | Plasma membrane | No |
| 61 | Tetraspanin 9 | 3 | O75954 | 4 | Multipass | Plasma membrane | No |
| 62 | Intestinal alkaline phosphatase | 3 | P09923 | 0 | GPI | Plasma membrane | No |
| 63 | Stabilin-1 | 3 | Q9NY15 | 1 | 1 | Plasma membrane | No |
| 64 | Kunitz-type protease inhibitor 2 | 2 | O43291 | 1 | 1 | Plasma membrane | No |
| 65 | Peptidyl-glycine α-amidating monooxygenase | 2 | P19021 | 1 | 1 | Plasma membrane | No |
| 66 | Platelet glycoprotein IX (GPIX) | 2 | P14770 | 1 | 1 | Plasma membrane | No |
| 67 | Ryanodine receptor 2 | 2 | Q92736 | 12 | Multipass | Plasma membrane | No |
| 68 | Stimulated by retinoic acid gene 6 (STRA6) protein homolog | 2 | Q9BX79 | 9 | Multipass | Plasma membrane | No |
| 69 | Transmembrane protein 56 | 2 | Q96MV1 | 6 | Multipass | Plasma membrane | No |
The search algorithms Sequest and Mascot were used to identify proteins that had 2 or more peptide hits with at least one of the algorithms. Proteins having a false positive identification rate of < 5% were selected. Transmembrane proteins were identified, and the subcellular location of the membrane protein was determined from the Swiss-Prot database search.
TMDs indicates transmembrane domains.
Shedding reported in platelets and myeloid cells.
Shedding reported in vascular endothelial cells and is ADAM17-mediated.
Shedding reported in Jurkat cells.
Shedding reported in endothelial and epithelial cells.