Primer sequences used for real-time PCR and ChIP assay
| Primer . | Use . | Sequence . | Accession no. . |
|---|---|---|---|
| EPO | Real-time PCR | 5′ctccgaacaatcactgct | X02158 |
| 3′ggtcatctgtcccctgtcct | |||
| Actin | Real-time PCR | 5′tcacccacaatgtgcccatctacga | X00351 |
| 3′cagcggaaccgctcattgccaatgg | |||
| HIF-1α | Real-time PCR | 5′gctggccccagccgctggag | AF208487 |
| 3′gagtgcagggtcagcactac | |||
| HIF-2α | Real-time PCR | 5′aggactacagcctgtcgtcagc | NM_001430 |
| 3′ccttgcaggagcgtggag | |||
| EPO 3′enhancer | ChIP assay | 5′ccaatatgaactcttggc | L16588 |
| 3′ctgcagccttgccctgggc | |||
| FSP-1 | qualitative PCR | 5′gagaaggccctggatgtgat | NM_002961 |
| 3′cctcgttgtccctgttgctg | |||
| CD73 | qualitative PCR | 5′cgcaacaatggcacaattat | NM_002526 |
| 3′ctcgacacttggtgcaaaga | |||
| PHD2 | Real-time PCR | 5′gcacgacaccgggaagtt | NM_022051 |
| 3′ccagcttcccgttacagt | |||
| PHD3 | Real-time PCR | 5′ggccatcagcttcctcctg | NM_022073.3 |
| 3′ggtgatgcagcgaccatca | |||
| Collagen IV | qualitative PCR | 5′gcgctggtgggtcagaaggc | NM_001845.4 |
| 3′ctgccttcaaggtggacggc |
| Primer . | Use . | Sequence . | Accession no. . |
|---|---|---|---|
| EPO | Real-time PCR | 5′ctccgaacaatcactgct | X02158 |
| 3′ggtcatctgtcccctgtcct | |||
| Actin | Real-time PCR | 5′tcacccacaatgtgcccatctacga | X00351 |
| 3′cagcggaaccgctcattgccaatgg | |||
| HIF-1α | Real-time PCR | 5′gctggccccagccgctggag | AF208487 |
| 3′gagtgcagggtcagcactac | |||
| HIF-2α | Real-time PCR | 5′aggactacagcctgtcgtcagc | NM_001430 |
| 3′ccttgcaggagcgtggag | |||
| EPO 3′enhancer | ChIP assay | 5′ccaatatgaactcttggc | L16588 |
| 3′ctgcagccttgccctgggc | |||
| FSP-1 | qualitative PCR | 5′gagaaggccctggatgtgat | NM_002961 |
| 3′cctcgttgtccctgttgctg | |||
| CD73 | qualitative PCR | 5′cgcaacaatggcacaattat | NM_002526 |
| 3′ctcgacacttggtgcaaaga | |||
| PHD2 | Real-time PCR | 5′gcacgacaccgggaagtt | NM_022051 |
| 3′ccagcttcccgttacagt | |||
| PHD3 | Real-time PCR | 5′ggccatcagcttcctcctg | NM_022073.3 |
| 3′ggtgatgcagcgaccatca | |||
| Collagen IV | qualitative PCR | 5′gcgctggtgggtcagaaggc | NM_001845.4 |
| 3′ctgccttcaaggtggacggc |