Effect of NAC treatment on MICA and MICB expression in T cells stimulated for 3 days with SEB
PBMCs were stimulated with SEB. After 48 hours, NAC was added to the cultures and left for an additional 18 hours. Cells were stained with specific mAbs plus GAM/PE and with anti–CD3/PECy5 mAb. NKG2DL expression was analyzed on CD3+CFSElow T cells. The MFI of isotypic control mAb was subtracted from the MFI of NKG2DL. The mean of MFI values of 6 different donors ± SD is shown. Statistical analysis was with the Student paired t test.
P < .05.
Not significant.