Primers used for genotyping of MHC class I U genes
| Gene . | Exon . | Primer pair . | Nucleotide sequence . | bp . |
|---|---|---|---|---|
| mhc1uxa2 | 2 | 1 | AGGCATACACTCTCTGAAATACTTCTTC | 264 |
| CCCTGTGACTGGTTAAACCTCTCC | ||||
| mhc1uea | 2 | 2 | TACACACTCCCTGAGGTACTTC | 204 |
| TGTGCGCCAGTAAAGATCTGAG | ||||
| mhc1uda | 2 | 1 | AGGCATACACTCTCTGAAATACTTCTTC | 264 |
| CCCTGTGACTGGTTAAACCTCTCC | ||||
| mhc1uda | 3 | 3 | GAAATGTACGGCTGTGAGTG | 252 |
| CAGGCTGCTCTTTCCATAC | ||||
| mhc1ufa | 2 | 4 | GAAAGCATTCTCACACTGTTATCT | 258 |
| GTGTTTGGTTAAAACGTTCCATTG | ||||
| mhc1ufa | 3 | 5 | CAGTTCATGGTCGGCTGTGA | 250 |
| AAGAATCTTTGCCGTAACCC | ||||
| LOC751750 | 2 | 6 | CACACCTGGAATGCCTATTACA | 245 |
| TTGAAGCGCTCTATTGCAACTT | ||||
| LOC751750 | 3 | 3 | GAAATGTACGGCTGTGAGTG | 252 |
| CAGGCTGCTCTTTCCATAC | ||||
| mhc1uba | 2 | 7 | CCACAGCTGGAAAGCCTATT | 266 |
| ATTTTTTACCTTGTGTTTGG | ||||
| mhc1uba | 3 | 8 | GTGTTCACACCTTCCAGTTC | 268 |
| TCCAAAGTGTCCTTCCCATA | ||||
| mhc1uca | 2 | 6 | CACACCTGGAATGCCTATTACA | 245 |
| TTGAAGCGCTCTATTGCAACTT | ||||
| mhc1uca | 3 | 9 | ACACATTACAGGAAATTTACGG | 260 |
| GCTGCTCTTTCCATACTCCAAG |
| Gene . | Exon . | Primer pair . | Nucleotide sequence . | bp . |
|---|---|---|---|---|
| mhc1uxa2 | 2 | 1 | AGGCATACACTCTCTGAAATACTTCTTC | 264 |
| CCCTGTGACTGGTTAAACCTCTCC | ||||
| mhc1uea | 2 | 2 | TACACACTCCCTGAGGTACTTC | 204 |
| TGTGCGCCAGTAAAGATCTGAG | ||||
| mhc1uda | 2 | 1 | AGGCATACACTCTCTGAAATACTTCTTC | 264 |
| CCCTGTGACTGGTTAAACCTCTCC | ||||
| mhc1uda | 3 | 3 | GAAATGTACGGCTGTGAGTG | 252 |
| CAGGCTGCTCTTTCCATAC | ||||
| mhc1ufa | 2 | 4 | GAAAGCATTCTCACACTGTTATCT | 258 |
| GTGTTTGGTTAAAACGTTCCATTG | ||||
| mhc1ufa | 3 | 5 | CAGTTCATGGTCGGCTGTGA | 250 |
| AAGAATCTTTGCCGTAACCC | ||||
| LOC751750 | 2 | 6 | CACACCTGGAATGCCTATTACA | 245 |
| TTGAAGCGCTCTATTGCAACTT | ||||
| LOC751750 | 3 | 3 | GAAATGTACGGCTGTGAGTG | 252 |
| CAGGCTGCTCTTTCCATAC | ||||
| mhc1uba | 2 | 7 | CCACAGCTGGAAAGCCTATT | 266 |
| ATTTTTTACCTTGTGTTTGG | ||||
| mhc1uba | 3 | 8 | GTGTTCACACCTTCCAGTTC | 268 |
| TCCAAAGTGTCCTTCCCATA | ||||
| mhc1uca | 2 | 6 | CACACCTGGAATGCCTATTACA | 245 |
| TTGAAGCGCTCTATTGCAACTT | ||||
| mhc1uca | 3 | 9 | ACACATTACAGGAAATTTACGG | 260 |
| GCTGCTCTTTCCATACTCCAAG |
The same pairs of primers were used for amplifying and sequencing.