Cytotoxicity of HTT and MG-132, alone and in combinations
| MG-132 alone, % . | HHT, 50nM . | HHT, 100nM . | |||
|---|---|---|---|---|---|
| Expected, % . | Observed, % . | Expected, % . | Observed, % . | ||
| MG-132, 0μM | 0 | 24 | 32 | ||
| MG-132, 1μM | 67 | 75 | 42 | 77 | 50 |
| MG-132, 10μM | 72 | 78 | 41 | 81 | 47 |
| MG-132, 100μM | 83 | 87 | 48 | 88 | 54 |
| MG-132 alone, % . | HHT, 50nM . | HHT, 100nM . | |||
|---|---|---|---|---|---|
| Expected, % . | Observed, % . | Expected, % . | Observed, % . | ||
| MG-132, 0μM | 0 | 24 | 32 | ||
| MG-132, 1μM | 67 | 75 | 42 | 77 | 50 |
| MG-132, 10μM | 72 | 78 | 41 | 81 | 47 |
| MG-132, 100μM | 83 | 87 | 48 | 88 | 54 |
CLL cells were treated with HHT or MG-132, alone or in combinations for 24 hours, cytotoxicity (percentage of cell death) was determined by annexin V–PI staining. Data represent the average of 3 independent experiments. The toxicities of the combination of HHT and MG-132 (observed) were compared with the expected cell death for an additive combination (expected).