Greater amplification of the modified erythroid cells with the LG/HA-Y1 than with the LG vector after extensive hematopoietic cell division in secondary transplants
| Group . | Primary . | Dilution . | WBC+ (theo) . | WBC+ . | RBC+ . | FE . |
|---|---|---|---|---|---|---|
| 2 | 30.8 | 5 | 6.2 | 2.1 | 9.5 | 5 |
| 2 | 24.7 | 7 | 3.5 | 1.0 | 7.7 | 8 |
| 2 | 31.6 | 10 | 3.2 | 8.8 | 54.0 | 12 |
| 2 | 9.4 | 4 | 2.4 | 3.5 | 0.0 | 0 |
| Mean | 3.8 | 3.9 | ||||
| SD | 1.7 | 3.5 | ||||
| 3 | 1.8 | 1 | 1.7 | 0.1 | 70.4 | 2374 |
| 3 | 3.1 | 1 | 3.1 | 1.2 | 55.2 | 102 |
| 3 | 25.2 | 4 | 5.7 | 9.8 | 88.2 | 69 |
| 3 | 24.8 | 2 | 11.7 | 16.0 | 90.1 | 48 |
| Mean | 5.6 | 6.8 | ||||
| SD | 4.4 | 7.5 |
| Group . | Primary . | Dilution . | WBC+ (theo) . | WBC+ . | RBC+ . | FE . |
|---|---|---|---|---|---|---|
| 2 | 30.8 | 5 | 6.2 | 2.1 | 9.5 | 5 |
| 2 | 24.7 | 7 | 3.5 | 1.0 | 7.7 | 8 |
| 2 | 31.6 | 10 | 3.2 | 8.8 | 54.0 | 12 |
| 2 | 9.4 | 4 | 2.4 | 3.5 | 0.0 | 0 |
| Mean | 3.8 | 3.9 | ||||
| SD | 1.7 | 3.5 | ||||
| 3 | 1.8 | 1 | 1.7 | 0.1 | 70.4 | 2374 |
| 3 | 3.1 | 1 | 3.1 | 1.2 | 55.2 | 102 |
| 3 | 25.2 | 4 | 5.7 | 9.8 | 88.2 | 69 |
| 3 | 24.8 | 2 | 11.7 | 16.0 | 90.1 | 48 |
| Mean | 5.6 | 6.8 | ||||
| SD | 4.4 | 7.5 |
Data are given for each secondary transplanted mouse with LG (group 2) and LG/HA-Y1 (group 3)-modified cells 10 weeks after secondary transplantation.
Dilution indicates dilution factor with cells from a mouse transplanted with mock-transduced cells; FE, amplification factor of modified erythroid cells vs modified leucocytes; primary, the percentage of modified nucleated cells in BM of primary donors; RBC+, percentage of RBCs containing human hemoglobin; WBC+ (theo), theoretical percentage of modified WBCs assuming 100% reconstitution with donor cells; WBC+, percentage of modified WBCs in secondary transplants assuming 100% reconstitution with donor cells and deduced from copy numbers.