Lineage potential of hematopoietic precursor cells
| Precursor cell . | Frequency of mature lineage colonies . | ||
|---|---|---|---|
| NK . | B . | T . | |
| NKP ID2-GFPhigh | 1:2.4 | 1:4681 | 1:7884 |
| 1:4.4 | 1:9462 | 1:7884 | |
| 1:2.4 | NC | NC | |
| 1:2.5 | ND | NC | |
| Mean NKP ID2-GFPhigh (95% CI) | 1:3.0 (2.6-3.6) | 1:6540 (2116-20 213) | 1:9448 (2494-39 673) |
| NKP ID2-GFPlow | 1:75 | 1:361 | 1:67 |
| 1:965 | 1:1821 | 1:172 | |
| 1:146.9 | 1:298 | 1:52.8 | |
| 1:129.7 | ND | 1:31.7 | |
| Mean NKP ID2-GFPlow (95% CI) | 1:337 (267-426) | 1:574 (409-805) | 1:138 (115-166) |
| CLP | 1:2.0 | 1:1.8 | 1:2.6 |
| 1:2.7 | 1:2.8 | ND | |
| 1:1.9 | ND | 1:1.8 | |
| 1:2.1 | 1:2.4 | ND | |
| 1:5.8 | 1:2.3 | ND | |
| 1:8.8 | 1:6.9 | ND | |
| Mean CLP (95% CI) | 1:2.4 (2.01-2.92) | 1:2.4 (1.91-2.97) | 1:2.1 (1.58-2.72) |
| pre-pro NK a | 1:1.6 | 1:239 | 1:113 |
| 1:3.4 | 1:159 | ND | |
| 1:1.4 | ND | 1:2046 | |
| 1:1.8 | 1:684 | ND | |
| 1:5.5 | 1:287 | ND | |
| pre-pro NK b | 1:1.8 | ND | 1:379 |
| 1:6.9 | 1:479 | ND | |
| Mean pre-pro NK (95% CI) | 1:2.4 (1.98-2.89) | 1:242 (150-392) | 1:416 (269-644) |
| Precursor cell . | Frequency of mature lineage colonies . | ||
|---|---|---|---|
| NK . | B . | T . | |
| NKP ID2-GFPhigh | 1:2.4 | 1:4681 | 1:7884 |
| 1:4.4 | 1:9462 | 1:7884 | |
| 1:2.4 | NC | NC | |
| 1:2.5 | ND | NC | |
| Mean NKP ID2-GFPhigh (95% CI) | 1:3.0 (2.6-3.6) | 1:6540 (2116-20 213) | 1:9448 (2494-39 673) |
| NKP ID2-GFPlow | 1:75 | 1:361 | 1:67 |
| 1:965 | 1:1821 | 1:172 | |
| 1:146.9 | 1:298 | 1:52.8 | |
| 1:129.7 | ND | 1:31.7 | |
| Mean NKP ID2-GFPlow (95% CI) | 1:337 (267-426) | 1:574 (409-805) | 1:138 (115-166) |
| CLP | 1:2.0 | 1:1.8 | 1:2.6 |
| 1:2.7 | 1:2.8 | ND | |
| 1:1.9 | ND | 1:1.8 | |
| 1:2.1 | 1:2.4 | ND | |
| 1:5.8 | 1:2.3 | ND | |
| 1:8.8 | 1:6.9 | ND | |
| Mean CLP (95% CI) | 1:2.4 (2.01-2.92) | 1:2.4 (1.91-2.97) | 1:2.1 (1.58-2.72) |
| pre-pro NK a | 1:1.6 | 1:239 | 1:113 |
| 1:3.4 | 1:159 | ND | |
| 1:1.4 | ND | 1:2046 | |
| 1:1.8 | 1:684 | ND | |
| 1:5.5 | 1:287 | ND | |
| pre-pro NK b | 1:1.8 | ND | 1:379 |
| 1:6.9 | 1:479 | ND | |
| Mean pre-pro NK (95% CI) | 1:2.4 (1.98-2.89) | 1:242 (150-392) | 1:416 (269-644) |
T-, B-, and NK-lineage potential within the NKP population and pre-pro NK-cell populations is shown. Limiting dilution analysis of NKPs (sorted as described in Figure 1 into ID2gfp-high and ID2gfp-low cells), CLPs, and pre-pro NK-cell subsets a and b (as described in Figure 1C) from Id2gfp/gfp BM. Sorted populations were cultured in either NK-, B-, or T-cell permissive conditions as described in “Methods.” Precursor frequency and 95% CIs were determined by the software application ELDA (ie, extreme limiting dilution analysis).13 Note the data from the pre-pro NK a and b populations has been combined for this analysis.
CI indicates confidence interval; CLP, common lymphoid progenitor; GFP, green fluorescent protein; ID2, inhibitor of DNA binding 2; NC, no colonies present; ND, not determined; NK, natural killer; and NKP, natural killer–cell progenitor.