Limiting dilution competitive reconstitution unit
| Donor genotype/cell dose . | Negative recipients, % (8 mice/group) . | CRU (95% CI) . |
|---|---|---|
| E47 WT | 1/10 974 (1/14 923, 1/7807) | |
| 200 000 | 0 | |
| 66 667 | 0 | |
| 22 222 | 12.5 | |
| 7778 | 50 | |
| E47 KO | 1/27 203 (1/36 769, 1/20 125) | |
| 200 000 | 0 | |
| 66 667 | 12.5 | |
| 22 222 | 37.5 | |
| 7778 | 75 |
| Donor genotype/cell dose . | Negative recipients, % (8 mice/group) . | CRU (95% CI) . |
|---|---|---|
| E47 WT | 1/10 974 (1/14 923, 1/7807) | |
| 200 000 | 0 | |
| 66 667 | 0 | |
| 22 222 | 12.5 | |
| 7778 | 50 | |
| E47 KO | 1/27 203 (1/36 769, 1/20 125) | |
| 200 000 | 0 | |
| 66 667 | 12.5 | |
| 22 222 | 37.5 | |
| 7778 | 75 |
The indicated doses of E47 WT or KO donor bone marrow cells were mixed with a constant dose (200 000 cells) of CD45.1 competitor WT bone marrow cells and transplanted into lethally irradiated CD45.1 recipient WT recipient mice. Blood reconstitution was examined at 16 weeks after adoptive transfer, and the threshold for positive engraftment was set as 1%.
CRU indicates competitive repopulation unit; and CI, confidence interval.