Table 1

Storage-related biochemical changes in rat platelet concentrates

Whole blood (fresh)Platelets
Day 0Day 5
pH 6.9 ± 0.1 7.4 ± 0.1 6.9 ± 0.1* 
Lactate, mmol/L 6.2 ± 1.2 8.0 ± 0.3 21.5 ± 1.2* 
Leukocytes, ×109/L 5.4 ± 0.6 0.03 ± 0.01 0.02 ± 0.01 
Platelets, ×109/L 858 ± 48 1086 ± 65 1076 ± 76 
Morphology, Kunicki score N/A 270 ± 8.7 192 ± 20.2† 
Percentage positive for annexin V N/A 4.3 ± 0.6 42 ± 1.5* 
LysoPC 16:0, μM  164 ± 13 183 ± 17† 
LysoPC 18:1, μM  41 ± 4.3 43 ± 1.6 
LysoPAF 16:0, μM  4.5 ± 0.4 4.9 ± 0.1 
LysoPAF 18:0, μM  5.1 ± 0.5 6.2 ± 0.4† 
LysoPAF 18:1, μM  2.0 ± 0.4 2.6 ± 0.4 
LysoPC 18:0/PAF 16:0, μM  5.1 ± 0.5 6.2 ± 0.4† 
PC 34:2, μM  86 ± 11 46 ± 4† 
PC 36:0, μM  23 ± 3.3 13 ± 0.9† 
TNF, pg/mL  < 62.5 < 62.5 
IL-6, pg/mL  < 31.25 < 31.25 
Whole blood (fresh)Platelets
Day 0Day 5
pH 6.9 ± 0.1 7.4 ± 0.1 6.9 ± 0.1* 
Lactate, mmol/L 6.2 ± 1.2 8.0 ± 0.3 21.5 ± 1.2* 
Leukocytes, ×109/L 5.4 ± 0.6 0.03 ± 0.01 0.02 ± 0.01 
Platelets, ×109/L 858 ± 48 1086 ± 65 1076 ± 76 
Morphology, Kunicki score N/A 270 ± 8.7 192 ± 20.2† 
Percentage positive for annexin V N/A 4.3 ± 0.6 42 ± 1.5* 
LysoPC 16:0, μM  164 ± 13 183 ± 17† 
LysoPC 18:1, μM  41 ± 4.3 43 ± 1.6 
LysoPAF 16:0, μM  4.5 ± 0.4 4.9 ± 0.1 
LysoPAF 18:0, μM  5.1 ± 0.5 6.2 ± 0.4† 
LysoPAF 18:1, μM  2.0 ± 0.4 2.6 ± 0.4 
LysoPC 18:0/PAF 16:0, μM  5.1 ± 0.5 6.2 ± 0.4† 
PC 34:2, μM  86 ± 11 46 ± 4† 
PC 36:0, μM  23 ± 3.3 13 ± 0.9† 
TNF, pg/mL  < 62.5 < 62.5 
IL-6, pg/mL  < 31.25 < 31.25 

Data are presented as mean ± SD (n = 5 batches).

IL-6 indicates interleukin-6; LysoPC, lysophosphatidylcholine; N/A, not available; PAF, platelet-activating factor; PC, phosphatidylcholine; and TNF, tumor necrosis factor.

*

P < .01.

†

P < .05.

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