Cytolytic and proliferative activity of human Vδ2neg γδ T-cell clones against B lymphoid target cells
| Clones* . | TCR† . | Daudi . | Raji . | RPMI 8226 . | B-LCL (Do#DA) . | T αβ§ . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Lysis‡ . | Proliferation . | Lysis . | Proliferation . | Lysis . | Proliferation . | Lysis . | Proliferation . | Lysis . | Proliferation . | ||
| 73R5 | Vγ8Vδ3 | 72 ± 1‖ | 1669 ± 6 | 52 ± 3‖ | 3453 ± 1325 | 7 ± 0 | 0 | 67 ± 12‖ | 29 389 ± 2479¶ | 14 ± 1 | 5887 ± 174 |
| 73R9 | Vγ8Vδ3 | 74 ± 2‖ | 18 889 ± 3156¶ | 50 ± 3‖ | 12 103 ± 824¶ | 3 ± 0 | 3317 ± 1081 | 80 ± 6‖ | 35 686 ± 2899¶ | 4 ± 6 | 825 ± 274 |
| 73R11 | Vγ8Vδ3 | 74 ± 0‖ | 12 815 ± 1598¶ | 50 ± 1‖ | 10 906 ± 436¶ | 7 ± 2 | 4324 ± 1300 | 86 ± 1‖ | 29 981 ± 902¶ | 7 ± 2 | 1894 ± 468 |
| 73R13 | Vγ8Vδ3 | 50 ± 8‖ | 192 ± 100 | 27 ± 1 | 4206 ± 1525 | 4 ± 2 | 3276 ± 1528 | 62 ± 3‖ | 2649 ± 768 | 7 ± 7 | 8551 ± 1173 |
| 73R14 | Vγ9Vδ1 | 73 ± 5‖ | 25 711 ± 274¶ | 35 ± 5‖ | 9963 ± 269 | 63 ± 5‖ | 3913 ± 200 | 12 ± 0 | 22 108 ± 395¶ | 12 ± 1 | 10 019 ± 730¶ |
| 84R11 | Vγ8Vδ1 | 1 ± 0 | 0 | 6 ± 2 | 0 | 15 ± 3 | 0 | 13 ± 3 | 12 102¶ | 6 ± 10 | 0 |
| 84R33 | Vγ3Vδ3 | 1 ± 0 | 0 | 2 ± 0 | 2905 ± 199 | 23 ± 5 | 2501 ± 656 | 1 ± 1 | 0 | 15 ± 6 | 3919 ± 50 |
| 84R45 | Vγ2-3Vδx | 0 | 0 | 3 ± 1 | 0 | 29 ± 1 | 0 | 4 ± 1 | 0 | 9 ± 10 | 0 |
| 84R50 | Vγ2-3Vδx | 1 ± 0 | 0 | 2 ± 0 | 3604 ± 74 | 39 ± 4‖ | 1312 ± 849 | 1 ± 3 | 0 | 7 ± 2 | 282 ± 174 |
| 84R51 | Vγ9Vδ2 | 64 ± 4‖ | ND | 9 ± 2 | ND | ND | ND | 5 ± 1 | ND | ND | ND |
| 84R48 | Vγ9Vδ2 | 63 ± 10‖ | ND | 4 ± 8 | ND | ND | ND | 15 ± 3 | ND | ND | ND |
| Clones* . | TCR† . | Daudi . | Raji . | RPMI 8226 . | B-LCL (Do#DA) . | T αβ§ . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Lysis‡ . | Proliferation . | Lysis . | Proliferation . | Lysis . | Proliferation . | Lysis . | Proliferation . | Lysis . | Proliferation . | ||
| 73R5 | Vγ8Vδ3 | 72 ± 1‖ | 1669 ± 6 | 52 ± 3‖ | 3453 ± 1325 | 7 ± 0 | 0 | 67 ± 12‖ | 29 389 ± 2479¶ | 14 ± 1 | 5887 ± 174 |
| 73R9 | Vγ8Vδ3 | 74 ± 2‖ | 18 889 ± 3156¶ | 50 ± 3‖ | 12 103 ± 824¶ | 3 ± 0 | 3317 ± 1081 | 80 ± 6‖ | 35 686 ± 2899¶ | 4 ± 6 | 825 ± 274 |
| 73R11 | Vγ8Vδ3 | 74 ± 0‖ | 12 815 ± 1598¶ | 50 ± 1‖ | 10 906 ± 436¶ | 7 ± 2 | 4324 ± 1300 | 86 ± 1‖ | 29 981 ± 902¶ | 7 ± 2 | 1894 ± 468 |
| 73R13 | Vγ8Vδ3 | 50 ± 8‖ | 192 ± 100 | 27 ± 1 | 4206 ± 1525 | 4 ± 2 | 3276 ± 1528 | 62 ± 3‖ | 2649 ± 768 | 7 ± 7 | 8551 ± 1173 |
| 73R14 | Vγ9Vδ1 | 73 ± 5‖ | 25 711 ± 274¶ | 35 ± 5‖ | 9963 ± 269 | 63 ± 5‖ | 3913 ± 200 | 12 ± 0 | 22 108 ± 395¶ | 12 ± 1 | 10 019 ± 730¶ |
| 84R11 | Vγ8Vδ1 | 1 ± 0 | 0 | 6 ± 2 | 0 | 15 ± 3 | 0 | 13 ± 3 | 12 102¶ | 6 ± 10 | 0 |
| 84R33 | Vγ3Vδ3 | 1 ± 0 | 0 | 2 ± 0 | 2905 ± 199 | 23 ± 5 | 2501 ± 656 | 1 ± 1 | 0 | 15 ± 6 | 3919 ± 50 |
| 84R45 | Vγ2-3Vδx | 0 | 0 | 3 ± 1 | 0 | 29 ± 1 | 0 | 4 ± 1 | 0 | 9 ± 10 | 0 |
| 84R50 | Vγ2-3Vδx | 1 ± 0 | 0 | 2 ± 0 | 3604 ± 74 | 39 ± 4‖ | 1312 ± 849 | 1 ± 3 | 0 | 7 ± 2 | 282 ± 174 |
| 84R51 | Vγ9Vδ2 | 64 ± 4‖ | ND | 9 ± 2 | ND | ND | ND | 5 ± 1 | ND | ND | ND |
| 84R48 | Vγ9Vδ2 | 63 ± 10‖ | ND | 4 ± 8 | ND | ND | ND | 15 ± 3 | ND | ND | ND |
ND indicates not determined.
γδ T-cell clones were derived from MLTC performed with PBL of 2 healthy human donors (Do#73 and Do#84), as described in “Cells.”
Expression of Vγ and Vδ TCR chains was determined by flow cytometry using specific mAbs.
Cytolytic activity (% of specific lysis) and γδ T-cell proliferation (cpm) were measured, respectively, in a standard 51Cr release (30:1 effector-to-target cell ratio) and 3H thymidine incorporation assays, as described in “Proliferation.” Data are shown as mean ± SD of triplicate (n = 3).
Control human CD8+ αβ T-cell line.
Cytolysis value > 30%.
Proliferation value > 10 000 cpm.