Table 2

Affymetrix microarray analysis of up- and down-regulated genes in EML-D816V cells compared with EML-Del417-419insY cells

GeneFold*
MC activation/differentiation-related genes  
    FceRI +66.64 
    Gp49a +30.35 
    MMCP-5 +16.78 
    MMCP-2 +3.03 
    Lgals3 +2.91 
    Spp1 +96.22 
    Gpr34 +9.57 
    Adora3 +20.81 
    Rab27b +15.58 
    Rab32 +3.68 
    C3ar1 +8.71 
    Mgst2 +5.81 
MC growth-related genes  
    Lif +16.12 
    Osm +3.99 
    Il3rb2 +3.01 
    Il4ra +4.6 
MC adhesion and migration-related genes  
    Tjp1 +31.46 
    Itgae +10.76 
    Ltb4r1 +4.03 
Suppressors of Epo signaling  
    Cis +17.14 
    Socs2 +13.99 
    Socs1 +4.81 
Erythroid cell-related genes  
    Ermap −3.28 
    Gypa −5.03 
    Ank −7.05 
    Spna1 −7.51 
GeneFold*
MC activation/differentiation-related genes  
    FceRI +66.64 
    Gp49a +30.35 
    MMCP-5 +16.78 
    MMCP-2 +3.03 
    Lgals3 +2.91 
    Spp1 +96.22 
    Gpr34 +9.57 
    Adora3 +20.81 
    Rab27b +15.58 
    Rab32 +3.68 
    C3ar1 +8.71 
    Mgst2 +5.81 
MC growth-related genes  
    Lif +16.12 
    Osm +3.99 
    Il3rb2 +3.01 
    Il4ra +4.6 
MC adhesion and migration-related genes  
    Tjp1 +31.46 
    Itgae +10.76 
    Ltb4r1 +4.03 
Suppressors of Epo signaling  
    Cis +17.14 
    Socs2 +13.99 
    Socs1 +4.81 
Erythroid cell-related genes  
    Ermap −3.28 
    Gypa −5.03 
    Ank −7.05 
    Spna1 −7.51 

Affymetrix microarrays were performed as described in Supplemental data. Gp49a indicates glycoprotein 49A; Lgals3, galectin-3; Spp1, secreted phosphoprotein 1; Gpr34, G protein–coupled receptor 34; Adora3, adenosine A3 receptor; Mgst2, microsomal glutathione S-transferase 2; Osm, oncostatin M; Tjp1, tight junction protein 1; Itgae, integrin α E; Ltb4r1, leukotriene B4 receptor 1; Ermap, erythroblast membrane-associated protein; Gypa, glycophorin A; Ank, progressive ankylosis; and Spnal, spectrin α 1.

*

Fold represents median gene expression level in two EML-D816V cells cultured for 48 or 72 hours relative to that in two EML-Del417−419insY cells. + indicates increase; and −, decrease. For all the cited genes, the P value was < .001.

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