Affymetrix microarray analysis of up- and down-regulated genes in EML-D816V cells compared with EML-Del417-419insY cells
Gene . | Fold* . |
---|---|
MC activation/differentiation-related genes | |
FceRI | +66.64 |
Gp49a | +30.35 |
MMCP-5 | +16.78 |
MMCP-2 | +3.03 |
Lgals3 | +2.91 |
Spp1 | +96.22 |
Gpr34 | +9.57 |
Adora3 | +20.81 |
Rab27b | +15.58 |
Rab32 | +3.68 |
C3ar1 | +8.71 |
Mgst2 | +5.81 |
MC growth-related genes | |
Lif | +16.12 |
Osm | +3.99 |
Il3rb2 | +3.01 |
Il4ra | +4.6 |
MC adhesion and migration-related genes | |
Tjp1 | +31.46 |
Itgae | +10.76 |
Ltb4r1 | +4.03 |
Suppressors of Epo signaling | |
Cis | +17.14 |
Socs2 | +13.99 |
Socs1 | +4.81 |
Erythroid cell-related genes | |
Ermap | −3.28 |
Gypa | −5.03 |
Ank | −7.05 |
Spna1 | −7.51 |
Gene . | Fold* . |
---|---|
MC activation/differentiation-related genes | |
FceRI | +66.64 |
Gp49a | +30.35 |
MMCP-5 | +16.78 |
MMCP-2 | +3.03 |
Lgals3 | +2.91 |
Spp1 | +96.22 |
Gpr34 | +9.57 |
Adora3 | +20.81 |
Rab27b | +15.58 |
Rab32 | +3.68 |
C3ar1 | +8.71 |
Mgst2 | +5.81 |
MC growth-related genes | |
Lif | +16.12 |
Osm | +3.99 |
Il3rb2 | +3.01 |
Il4ra | +4.6 |
MC adhesion and migration-related genes | |
Tjp1 | +31.46 |
Itgae | +10.76 |
Ltb4r1 | +4.03 |
Suppressors of Epo signaling | |
Cis | +17.14 |
Socs2 | +13.99 |
Socs1 | +4.81 |
Erythroid cell-related genes | |
Ermap | −3.28 |
Gypa | −5.03 |
Ank | −7.05 |
Spna1 | −7.51 |
Affymetrix microarrays were performed as described in Supplemental data. Gp49a indicates glycoprotein 49A; Lgals3, galectin-3; Spp1, secreted phosphoprotein 1; Gpr34, G protein–coupled receptor 34; Adora3, adenosine A3 receptor; Mgst2, microsomal glutathione S-transferase 2; Osm, oncostatin M; Tjp1, tight junction protein 1; Itgae, integrin α E; Ltb4r1, leukotriene B4 receptor 1; Ermap, erythroblast membrane-associated protein; Gypa, glycophorin A; Ank, progressive ankylosis; and Spnal, spectrin α 1.
Fold represents median gene expression level in two EML-D816V cells cultured for 48 or 72 hours relative to that in two EML-Del417−419insY cells. + indicates increase; and −, decrease. For all the cited genes, the P value was < .001.