Primers used in PCR
| Gene amplified/primer . | Sequence, 5′ → 3′ . |
|---|---|
| MC-CPA | |
| Sense | GGACTGTGGCATTCATGCACG |
| Antisense | CATCCGTGGCAATCCTTGCAA |
| CD25 | |
| Sense | GACTTCCCACAACCCACAGAAACA |
| Antisense | AGGAAACAGCCGTTAGGTGAATGC |
| β-actin | |
| Sense | GTACCACAGGCATTGTGATGGACT |
| Antisense | CTTTGATGTCACGCACGATTTCCC |
| Gene amplified/primer . | Sequence, 5′ → 3′ . |
|---|---|
| MC-CPA | |
| Sense | GGACTGTGGCATTCATGCACG |
| Antisense | CATCCGTGGCAATCCTTGCAA |
| CD25 | |
| Sense | GACTTCCCACAACCCACAGAAACA |
| Antisense | AGGAAACAGCCGTTAGGTGAATGC |
| β-actin | |
| Sense | GTACCACAGGCATTGTGATGGACT |
| Antisense | CTTTGATGTCACGCACGATTTCCC |