ECP-enhanced expression of DC marker genes ratio* of ECP D1/pre-ECP levels
| Gene . | Attributes . | CTCL and GVHD (N = 6) induced expression ratio . | Normal subjects (N = 6) induced expression ratio . |
|---|---|---|---|
| DC-LAMP | DC lysomal protein, role in class II antigen processing.17 | 27.6 | 17.2 |
| P = 1.2 × 10−9 | P = 1.4 × 10−7 | ||
| GPNMB | Transmembrane glycoprotein containing the integrin-binding motif RGD.18 | 205.7 | 123.3 |
| P = 9.6 × 10−15 | P = 2.8 × 10−14 | ||
| CD80 | Costimulatory molecule, B7.1 activates T cells.19 | 13.4 | NC |
| P = 2.3 × 10−13 | |||
| CD86 | Costimulatory molecule, B7.2 activates T cells.19 | NC | 50P = 1.4×10−5 |
| CD40 | Prolongs B7.1 and 2 T-cell activation.19 | 2.3 | NC |
| P = 5.7 × 10−4 | |||
| Decysin | Expressed in LPS-matured DCs but not monocytes or immature DCs.20 | 26.5 | 7.1 |
| P = 1.0 × 10−9 | P = 5.6 × 10−4 | ||
| CCR7 | Facilitates DC entry into lymph nodes where antigen presentation to naïve T cells occurs.21 | 2.6P = 7.0 × 10−3 | NC |
| CD83 | DC maturation molecule.11-13 | NC | 2.3 |
| P = .03 | |||
| OLR1 | Lox1, lectin-like receptor expressed on DCs but absent from monocytes.25 | 13.6 | 100.1 |
| P = 3.3 × 10−5 | P = 8.3 × 10−8 | ||
| FPRL2 | Formyl peptide receptor-like-2 expressed on mature DCs and involved in trafficking | 33.9 | 43.2 |
| and T-cell stimulation.26 | P = 2.1 × 10−8 | P = 1.9 × 10−8 |
| Gene . | Attributes . | CTCL and GVHD (N = 6) induced expression ratio . | Normal subjects (N = 6) induced expression ratio . |
|---|---|---|---|
| DC-LAMP | DC lysomal protein, role in class II antigen processing.17 | 27.6 | 17.2 |
| P = 1.2 × 10−9 | P = 1.4 × 10−7 | ||
| GPNMB | Transmembrane glycoprotein containing the integrin-binding motif RGD.18 | 205.7 | 123.3 |
| P = 9.6 × 10−15 | P = 2.8 × 10−14 | ||
| CD80 | Costimulatory molecule, B7.1 activates T cells.19 | 13.4 | NC |
| P = 2.3 × 10−13 | |||
| CD86 | Costimulatory molecule, B7.2 activates T cells.19 | NC | 50P = 1.4×10−5 |
| CD40 | Prolongs B7.1 and 2 T-cell activation.19 | 2.3 | NC |
| P = 5.7 × 10−4 | |||
| Decysin | Expressed in LPS-matured DCs but not monocytes or immature DCs.20 | 26.5 | 7.1 |
| P = 1.0 × 10−9 | P = 5.6 × 10−4 | ||
| CCR7 | Facilitates DC entry into lymph nodes where antigen presentation to naïve T cells occurs.21 | 2.6P = 7.0 × 10−3 | NC |
| CD83 | DC maturation molecule.11-13 | NC | 2.3 |
| P = .03 | |||
| OLR1 | Lox1, lectin-like receptor expressed on DCs but absent from monocytes.25 | 13.6 | 100.1 |
| P = 3.3 × 10−5 | P = 8.3 × 10−8 | ||
| FPRL2 | Formyl peptide receptor-like-2 expressed on mature DCs and involved in trafficking | 33.9 | 43.2 |
| and T-cell stimulation.26 | P = 2.1 × 10−8 | P = 1.9 × 10−8 |
Fold increase in expression of multiple genes involved in DC maturation and function induced by ECP.
Impact of treatment on gene expression is displayed as an induced expression ratio (ratio of ECP Day 1 to pre-ECP expression for the relevant gene). RNA was isolated from 3 CTCL patients and 3 GVHD patients and 6 normal subjects at the relevant time points.