Analysis of NHR2-m5 oligomerization by sedimentation velocity analysis
| Concentration . | S, Svedbergs . | Mass,* kDa . | f/f0† . | r.m.s.‡ . | Percentage contribution§ . |
|---|---|---|---|---|---|
| 15μM | 2.80 | 25.0 | 1.07 | 4.7 × 10−3 | 77 |
| 30μM | 2.75 | 23.0 | 1.09 | 8.0 × 10−3 | 86 |
| Concentration . | S, Svedbergs . | Mass,* kDa . | f/f0† . | r.m.s.‡ . | Percentage contribution§ . |
|---|---|---|---|---|---|
| 15μM | 2.80 | 25.0 | 1.07 | 4.7 × 10−3 | 77 |
| 30μM | 2.75 | 23.0 | 1.09 | 8.0 × 10−3 | 86 |
Sedimentation (S) profile for the NHR2-m5 protein at 2 different loading concentrations. Data were analyzed using the c(s) continuous distribution of Lamm equation solutions with SEDFIT and SEDPHAT.
The molecular mass estimation was obtained using the optimal frictional ratio (f/f0) with SEDFIT.
The ratio f/f0 is the frictional coefficient of a particle of a given axial ratio divided by the frictional coefficient of a sphere of the same volume.
Root-mean-square (r.m.s.) deviation of the global fit in units of absorbance (OD).
The weight percentage contribution of the majority species was obtained by integration of a continuous sedimentation coefficient distribution from approximately 1.5 to approximately 3.5 S with SEDFIT.