Variables with P values of less than or equal to .1 in univariate analysis were included for the multivariate analysis. For OS, these included age, FLT3-ITD, WT1 expression, extramedullary disease, platelets, NPM1/FLT3 status low risk vs others, CEBPA mutation, and WT1 SNP rs16754. For RFS, these included CEBPA mutation, WT1 SNP rs16754, NPM1 mutation, and NPM1/FLT3 status low risk vs others. Variables evaluated in univariate analysis (see also supplemental Table 3) included FLT3-ITD, age, WT1 expression, extramedullary disease, platelets high vs low, NPM1/FLT3 status low risk vs others, CEBPA mutation, WT1 SNP rs16754, NPM1 mutation, WT1 mutation, sibling vs no sibling donor, white blood cells high vs low, peripheral blasts high vs low, hemoglobin high vs low, de novo vs secondary AML, sex, IDH2 mutation, and IDH1 or IDH2 mutation combined.

HR indicates hazard ratio; and CI, confidence interval.

NPM1/FLT3 low risk is defined as the presence of an NPM1 mutation in the absence of an FLT3-ITD.