Peptide ligands isolated from phage-display libraries
| Phage clones . | Sequence . | Absorbance* . |
|---|---|---|
| f88-C7C library | ||
| A20-2 | CSSLVWYRC | 0.45 |
| A20-5 | CKSSSPIIC | 0.88 |
| A20-6 | CKSSSPIVC | 1.20 |
| A20-12 | CTLTPWYMC | 0.55 |
| A20-28 | CHTQKASVC | 0.38 |
| A20-60 | CQPWYSGLC | 0.39 |
| f88-12 library | ||
| A20-1 | QVYKQSGSPVYF | 1.15 |
| A20-3 | HYCTSPVVASYA | 0.66 |
| A20-9 | QVYKQSGSPVYF | 1.05 |
| A20-13 | STLPWYQQLSAP | 0.35 |
| A20-15 | NFPIRAWYQDVS | 0.43 |
| A20-41 | QVYKQSGPSVYF | 0.58 |
| A20-42 | QVYKQSGPSVYF | 0.55 |
| A20-43 | QVYKQSGPSVYF | 0.55 |
| f88-Cys6 | ||
| A20-7 | ENPICELSKQECDFWT | 0.73 |
| A20-17 | DRGGCKLSVNGCVGNG | 0.69 |
| A20-36 | EYVNCDNLVGNCVIRG | 1.88 |
| A20-37 | EYVNCDNLVGNCVIRG | 1.65 |
| A20-44 | EYVNCDNLVGNCVIRG | 1.78 |
| A20-45 | EYVNCDNLVGNCVIRG | 1.80 |
| A20-46 | EYVNCDNLVGNCVIRG | 1.78 |
| A20-52 | EYVNCDNLVGNCVIRG | 1.80 |
| Wild-type | 0.04 |
| Phage clones . | Sequence . | Absorbance* . |
|---|---|---|
| f88-C7C library | ||
| A20-2 | CSSLVWYRC | 0.45 |
| A20-5 | CKSSSPIIC | 0.88 |
| A20-6 | CKSSSPIVC | 1.20 |
| A20-12 | CTLTPWYMC | 0.55 |
| A20-28 | CHTQKASVC | 0.38 |
| A20-60 | CQPWYSGLC | 0.39 |
| f88-12 library | ||
| A20-1 | QVYKQSGSPVYF | 1.15 |
| A20-3 | HYCTSPVVASYA | 0.66 |
| A20-9 | QVYKQSGSPVYF | 1.05 |
| A20-13 | STLPWYQQLSAP | 0.35 |
| A20-15 | NFPIRAWYQDVS | 0.43 |
| A20-41 | QVYKQSGPSVYF | 0.58 |
| A20-42 | QVYKQSGPSVYF | 0.55 |
| A20-43 | QVYKQSGPSVYF | 0.55 |
| f88-Cys6 | ||
| A20-7 | ENPICELSKQECDFWT | 0.73 |
| A20-17 | DRGGCKLSVNGCVGNG | 0.69 |
| A20-36 | EYVNCDNLVGNCVIRG | 1.88 |
| A20-37 | EYVNCDNLVGNCVIRG | 1.65 |
| A20-44 | EYVNCDNLVGNCVIRG | 1.78 |
| A20-45 | EYVNCDNLVGNCVIRG | 1.80 |
| A20-46 | EYVNCDNLVGNCVIRG | 1.78 |
| A20-52 | EYVNCDNLVGNCVIRG | 1.80 |
| Wild-type | 0.04 |
Peptide insert sequences of phage clones isolated from 3 RPLs by affinity purification with A20-Ig.
ELISA absorbance values of single phage clones were expressed as the difference between OD405nm and OD620nm.