Summary of results from mice treated for 3 days with indicated minipump or 10 days with indicated IL-4C treatment
| Recipient . | Dose . | Liver . | Spleen . | Bone marrow . | ||
|---|---|---|---|---|---|---|
| YM1+ Kupffer cell hyperplasia; endothelial cell adhesion . | Erythrophagocytosis . | Erythrophagocytosis . | Histiocytosis, YM1+ macrophages . | Erythrophagocytosis . | ||
| B6 WT | IL-4 3 μg/hour minipump | ++ | ++ | + | ++ | ND |
| IL-4 1 μg/hour minipump | ++ | +++ | + | ++ | ++ | |
| IL-13 1 μg/hour minipump | + | − | − | − | − | |
| IL-4 1 μg/hour minipump + anti-IFNγ | ++ | +++ | + | ++ | ++ | |
| anti-interferon-γ alone | − | − | − | − | ND | |
| IL-4C + anti-interferon-γ | ++ | ++ | − | ++ | ND | |
| IL-4C alone | ++ | ++ | − | + | ND | |
| B6 Rag2−/− | IL-4 3 μg/hour minipump | +++ | ++ | − | ++ | ++ |
| IL-4 1 μg/hour | ++ | ++ | − | ++ | ++ | |
| IL-13 3 μg/hour minipump | + | − | − | − | + | |
| IL-13 1 μg/hour minipump | + | − | − | − | − | |
| B6 Stat6−/− | IL-4 1 μg/hour minipump | − | +/− | − | − | − |
| Recipient . | Dose . | Liver . | Spleen . | Bone marrow . | ||
|---|---|---|---|---|---|---|
| YM1+ Kupffer cell hyperplasia; endothelial cell adhesion . | Erythrophagocytosis . | Erythrophagocytosis . | Histiocytosis, YM1+ macrophages . | Erythrophagocytosis . | ||
| B6 WT | IL-4 3 μg/hour minipump | ++ | ++ | + | ++ | ND |
| IL-4 1 μg/hour minipump | ++ | +++ | + | ++ | ++ | |
| IL-13 1 μg/hour minipump | + | − | − | − | − | |
| IL-4 1 μg/hour minipump + anti-IFNγ | ++ | +++ | + | ++ | ++ | |
| anti-interferon-γ alone | − | − | − | − | ND | |
| IL-4C + anti-interferon-γ | ++ | ++ | − | ++ | ND | |
| IL-4C alone | ++ | ++ | − | + | ND | |
| B6 Rag2−/− | IL-4 3 μg/hour minipump | +++ | ++ | − | ++ | ++ |
| IL-4 1 μg/hour | ++ | ++ | − | ++ | ++ | |
| IL-13 3 μg/hour minipump | + | − | − | − | + | |
| IL-13 1 μg/hour minipump | + | − | − | − | − | |
| B6 Stat6−/− | IL-4 1 μg/hour minipump | − | +/− | − | − | − |
Three to 5 mice/group were treated, and histopathology evaluated in at least 2 separate experiments. Histiocytosis: 0 indicates within normal limits; +, minimal; ++, moderate; +++, severe. Hematopoiesis: 0, within normal limits; +, minimal; ++, mild; +++, moderate; ++++, extensive. Erythrophagocytosis: 0, not observed; +, 1 erythrophagocytic cell per high power field (hpf/40×); ++, 2 erythrophagocytic cells/hpf; +++, 3 erythrophagocytic cells/hpf. ND, not done.