Capture efficiency and sequencing statistics for WES results
| . | DNA pool #1 . | DNA pool #2 . | DNA pool #3 . | DNA pool #4 . |
|---|---|---|---|---|
| Total read (k) | 205 303 | 205 335 | 200 960 | 209 335 |
| After trimmed (k) | 203 977 | 204 017 | 198 493 | 206 924 |
| Genome mapped (k) | 180 133 | 179 889 | 175 683 | 181 008 |
| On target (k) | 129 112 | 133 493 | 111 209 | 128 144 |
| Mean coverage (×) | 179.6 | 188.0 | 157.5 | 163.7 |
| % on target at 100× coverage | 62.73 | 66.30 | 59.86 | 61.21 |
| Pearson correlation with SNP 6.0 | 0.985 | 0.983 | 0.983 | 0.984 |
| . | DNA pool #1 . | DNA pool #2 . | DNA pool #3 . | DNA pool #4 . |
|---|---|---|---|---|
| Total read (k) | 205 303 | 205 335 | 200 960 | 209 335 |
| After trimmed (k) | 203 977 | 204 017 | 198 493 | 206 924 |
| Genome mapped (k) | 180 133 | 179 889 | 175 683 | 181 008 |
| On target (k) | 129 112 | 133 493 | 111 209 | 128 144 |
| Mean coverage (×) | 179.6 | 188.0 | 157.5 | 163.7 |
| % on target at 100× coverage | 62.73 | 66.30 | 59.86 | 61.21 |
| Pearson correlation with SNP 6.0 | 0.985 | 0.983 | 0.983 | 0.984 |
The DNA pools contained 52 control patients in DNA pool #1; 52 control patients in DNA pool #2; 60 patients with stroke in DNA pool #3; and 60 patients with stroke in DNA pool #4. At >100× coverage, ∼60% of all sequences were on target for each of the DNA pools. Compared with the individual genotypes obtained from the SNP 6.0 arrays, a strong correlation with genotype data was generated by WES for each of the DNA pools.