Limiting dilution analysis of NOD/Lt-scid and NSG mice
| Genotype/group . | Frequency of SRCs* . | Pearson P† . | |
|---|---|---|---|
| Mean . | 95% CI . | ||
| NOD/Lt-scid | |||
| Female | 1/8801 | 4424-17 509 | .472 |
| Male | 1/2545 | 891-7268 | .367 |
| Both | 1/6497 | 3781-11 166 | .097 |
| NSG | |||
| Female | 1/964 | 444-2095 | .209 |
| Male | 1/2767 | 1379-5553 | .371 |
| Both | 1/1799 | 1076-3008 | .023 |
| Genotype/group . | Frequency of SRCs* . | Pearson P† . | |
|---|---|---|---|
| Mean . | 95% CI . | ||
| NOD/Lt-scid | |||
| Female | 1/8801 | 4424-17 509 | .472 |
| Male | 1/2545 | 891-7268 | .367 |
| Both | 1/6497 | 3781-11 166 | .097 |
| NSG | |||
| Female | 1/964 | 444-2095 | .209 |
| Male | 1/2767 | 1379-5553 | .371 |
| Both | 1/1799 | 1076-3008 | .023 |
Eight- to 9-week-old female and male NOD/Lt-scid and NSG mice were injected in the RF at limiting doses with Lin− CB cells (500, 2500, 7500, or 20 000), and engraftment was measured 13 weeks later.
Frequency of SRCs in the RF was calculated using L-Calc software (StemCell Technologies) with the number of positively engrafted mice at each dose.
Pearson P value to test for inconsistency.