Engraftment efficiency of tissues and strains
| Tissue/genotype . | No. engrafted mice*/total . | |||
|---|---|---|---|---|
| Experiment 1 . | Experiment 2 . | Combined . | Percentage . | |
| RF | ||||
| NOD/Lt-scid | 10/11† | 5/7 | 15/18 | 83.3 |
| NOD/Shi-scid | 12/12 | 3/11 | 15/23 | 65.2 |
| NSG | 12/12 | 11/11 | 23/23 | 100 |
| NOG | 11/12 | 9/10 | 20/22 | 90.9 |
| BM | ||||
| NOD/Lt-scid | 9/11 | 5/7 | 14/18 | 77.8 |
| NOD/Shi-scid | 12/12 | 3/11 | 15/23 | 65.2 |
| NSG | 12/12 | 11/11 | 23/23 | 100 |
| NOG | 10/12 | 9/10 | 19/22 | 86.4 |
| Spleen | ||||
| NOD/Lt-scid | 9/11 | 4/7 | 13/18 | 72.2 |
| NOD/Shi-scid | 12/12 | 3/11 | 15/23 | 65.2 |
| NSG | 12/12 | 11/11 | 23/23 | 100 |
| NOG | 10/12 | 9/10 | 19/22 | 86.4 |
| Thymus | ||||
| NOD/Lt-scid | 0/9‡ | 0/7 | 0/16 | 0 |
| NOD/Shi-scid | 3/10‡ | 1/11 | 4/21 | 19.0 |
| NSG | 4/7‡ | 10/11 | 14/18 | 77.8 |
| NOG | 5/11‡ | 7/10 | 12/21 | 57.1 |
| Tissue/genotype . | No. engrafted mice*/total . | |||
|---|---|---|---|---|
| Experiment 1 . | Experiment 2 . | Combined . | Percentage . | |
| RF | ||||
| NOD/Lt-scid | 10/11† | 5/7 | 15/18 | 83.3 |
| NOD/Shi-scid | 12/12 | 3/11 | 15/23 | 65.2 |
| NSG | 12/12 | 11/11 | 23/23 | 100 |
| NOG | 11/12 | 9/10 | 20/22 | 90.9 |
| BM | ||||
| NOD/Lt-scid | 9/11 | 5/7 | 14/18 | 77.8 |
| NOD/Shi-scid | 12/12 | 3/11 | 15/23 | 65.2 |
| NSG | 12/12 | 11/11 | 23/23 | 100 |
| NOG | 10/12 | 9/10 | 19/22 | 86.4 |
| Spleen | ||||
| NOD/Lt-scid | 9/11 | 4/7 | 13/18 | 72.2 |
| NOD/Shi-scid | 12/12 | 3/11 | 15/23 | 65.2 |
| NSG | 12/12 | 11/11 | 23/23 | 100 |
| NOG | 10/12 | 9/10 | 19/22 | 86.4 |
| Thymus | ||||
| NOD/Lt-scid | 0/9‡ | 0/7 | 0/16 | 0 |
| NOD/Shi-scid | 3/10‡ | 1/11 | 4/21 | 19.0 |
| NSG | 4/7‡ | 10/11 | 14/18 | 77.8 |
| NOG | 5/11‡ | 7/10 | 12/21 | 57.1 |
Eight- to 13-week-old female and male mice were injected with 2.3 × 104 (experiment 1) or 3.0 × 104 (experiment 2) Lin− CB cells (n = 5-7 per sex).
Positive engraftment defined using isotype controls in addition to staining for lineage markers.
Number of mice engrafted with CD45+ cells/total mice.
Thymic tissue was not assayed because of limited tissue availability for some mice in experiment 1.