Expression of type I IFN-regulated genes in platelets from SLE patients in comparison with healthy controls given as fold change
| Gene symbol . | Gene title . | Fold change . |
|---|---|---|
| IFI27 | interferon, alpha-inducible protein 27 | 23.12 |
| CD58* | CD58 antigen, (lymphocyte function-associated antigen 3) | 13.04 |
| PRKRA* | protein kinase, interferon-inducible double stranded RNA dependent activator | 6.34 |
| G1P3 | interferon, alpha-inducible protein (clone IFI-6-16) | 6.27 |
| CD69* | CD69 antigen (p60, early T-cell activation antigen) | 5.25 |
| IFITM1* | interferon induced transmembrane protein 1 (9-27) | 3.42 |
| OAS1 | 2′,5′-oligoadenylate synthetase 1, 40/46kDa | 2.87 |
| STAT1 | signal transducer and activator of transcription 1, 91kDa | 2.58 |
| LY6E | lymphocyte antigen 6 complex, locus E | 2.43 |
| IFNGR1 | interferon gamma receptor 1 | 2.18 |
| IFRD1 | interferon-related developmental regulator 1 | 2.10 |
| IFI44L | interferon-induced protein 44-like | 2.04 |
| OAS3 | 2′-5′-oligoadenylate synthetase 3, 100kDa | 1.98 |
| G1P2 | interferon, alpha-inducible protein (clone IFI-15K) | 1.98 |
| PLSCR1 | phospholipid scramblase 1 | 1.97 |
| OAS2 | 2′-5′-oligoadenylate synthetase 2, 69/71kDa | 1.92 |
| IRF2BP2 | interferon regulatory factor 2 binding protein 2 | 1.92 |
| GBP1 | guanylate binding protein 1, interferon-inducible, 67kDa | 1.87 |
| IFI16 | interferon, gamma-inducible protein 16 | 1.83 |
| IFITM3 | interferon induced transmembrane protein 3 (1-8U) | 1.80 |
| IRF2 | interferon regulatory factor 2 | 1.70 |
| Gene symbol . | Gene title . | Fold change . |
|---|---|---|
| IFI27 | interferon, alpha-inducible protein 27 | 23.12 |
| CD58* | CD58 antigen, (lymphocyte function-associated antigen 3) | 13.04 |
| PRKRA* | protein kinase, interferon-inducible double stranded RNA dependent activator | 6.34 |
| G1P3 | interferon, alpha-inducible protein (clone IFI-6-16) | 6.27 |
| CD69* | CD69 antigen (p60, early T-cell activation antigen) | 5.25 |
| IFITM1* | interferon induced transmembrane protein 1 (9-27) | 3.42 |
| OAS1 | 2′,5′-oligoadenylate synthetase 1, 40/46kDa | 2.87 |
| STAT1 | signal transducer and activator of transcription 1, 91kDa | 2.58 |
| LY6E | lymphocyte antigen 6 complex, locus E | 2.43 |
| IFNGR1 | interferon gamma receptor 1 | 2.18 |
| IFRD1 | interferon-related developmental regulator 1 | 2.10 |
| IFI44L | interferon-induced protein 44-like | 2.04 |
| OAS3 | 2′-5′-oligoadenylate synthetase 3, 100kDa | 1.98 |
| G1P2 | interferon, alpha-inducible protein (clone IFI-15K) | 1.98 |
| PLSCR1 | phospholipid scramblase 1 | 1.97 |
| OAS2 | 2′-5′-oligoadenylate synthetase 2, 69/71kDa | 1.92 |
| IRF2BP2 | interferon regulatory factor 2 binding protein 2 | 1.92 |
| GBP1 | guanylate binding protein 1, interferon-inducible, 67kDa | 1.87 |
| IFI16 | interferon, gamma-inducible protein 16 | 1.83 |
| IFITM3 | interferon induced transmembrane protein 3 (1-8U) | 1.80 |
| IRF2 | interferon regulatory factor 2 | 1.70 |
The genes were selected based on type I IFN-regulated genes reported up-regulated in SLE patients' peripheral blood mononuclear cells and also several other type I IFN-associated genes as determined by the open access database INTERFEROME (http://www.interferome.org). All microarray data are available in supplemental Table 1 and at GEO under accession number GSE22132.
IFN indicates interferon; and SLE, systemic lupus erythematosus.
Expression of genes was further validated by real-time polymerase chain reaction and flow cytometry.