Table 2

Predicted PKA substrates

IPIProteinSite1Site2Site310 minutes
60 minutes
RatioSTDEVRatioSTDEV
IPI00289608 AP3D1, Isoform 2 of AP-3 complex subunit δ-1 S567   16.87 4.08 1.84 0.58 
IPI00418471 VIM, Vimentin S39   12.63 3.75 2.06 0.61 
IPI00102670 FNBP1, Isoform 2 of Formin-binding protein 1 S296 S301  11.79 1.47 1.13 0.04 
IPI00009334 PRKD2, Serine/threonine-protein kinase D2 S197 S198  10.43 NaN 2.37 NaN 
IPI00021389 CCS, Copper chaperone for superoxide dismutase S267   9.63 NaN 2.68 NaN 
IPI00004407 SIT1, Signaling threshold-regulating transmembrane adapter 1 S182   9.21 NaN 2.24 NaN 
IPI00791144 EPB41, EPB41 protein S549   8.66 NaN 2.52 NaN 
IPI00008994 NDRG2, Isoform 1 of protein NDRG2 S332   8.38 0.90 1.95 0.24 
IPI00418471 VIM, Vimentin S25   8.31 NaN 1.83 NaN 
IPI00292228 GSK3A, Glycogen synthase kinase-3 α S21   7.95 NaN 2.08 NaN 
IPI00789551 MATR3, Uncharacterized protein MATR3 T150   7.47 NaN 4.00 NaN 
IPI00410067 ZC3HAV1, Isoform 1 of zinc finger CCCH-type antiviral protein 1 S284   7.41 NaN 3.91 NaN 
IPI00410067 ZC3HAV1, Isoform 1 of zinc finger CCCH-type antiviral protein 1 S284   7.30 NaN 3.31 NaN 
IPI00014256 ARHGEF6, Isoform 1 of Rho guanine nucleotide exchange factor 6 S640   7.06 NaN 2.35 NaN 
IPI00027430 SPN, Leukosialin precursor T341   6.93 NaN 1.94 NaN 
IPI00007722 AMPD2, Adenosine monophosphate deaminase 2 S76   6.10 NaN 1.91 NaN 
IPI00301058 VASP, Vasodilator-stimulated phosphoprotein S239   6.03 0.39 1.84 0.18 
IPI00022449 DOCK2, Isoform 1 of dedicator of cytokinesis protein 2 S1706   5.78 NaN 1.63 NaN 
IPI00217393 CENTG1, Isoform 1 of centaurin-γ-1 S648   5.72 0.42 1.81 0.10 
IPI00026928 GRAP2, GRB2-related adapter protein 2 T262   5.29 1.87 2.61 0.83 
IPI00418471 VIM, Vimentin S73   4.54 1.28 2.68 0.90 
IPI00442053 ARHGAP9, Isoform 2 of Rho GTPase-activating protein 9 S481   4.47 0.24 1.61 0.09 
IPI00013174 RBM14, Isoform 1 of RNA-binding protein 14 S618   4.26 NaN 2.66 NaN 
IPI00028065 NCK1, Cytoplasmic protein NCK1 S85   4.24 NaN 1.85 NaN 
IPI00329495 ABLIM1, Isoform 1 of actin-binding LIM protein 1 S587   4.05 1.28 1.57 0.43 
IPI00785100 MEF2D, Myocyte enhancer factor 2D S121   4.01 NaN 1.86 NaN 
IPI00032355 PUM1, Pumilio homolog 1 S745   3.68 NaN 1.61 NaN 
IPI00171176 PANK2, Isoform 1 of pantothenate kinase 2, mitochondrial prec. S168   3.26 NaN 1.36 NaN 
IPI00297160 CD44, Isoform 12 of CD44 antigen precursor S316   2.90 NaN 2.66 NaN 
IPI00028027 GSDMDC1, Gasdermin domain-containing protein 1 S185   2.88 NaN 1.52 NaN 
IPI00418471 VIM, Vimentin S66   2.84 0.21 1.15 0.10 
IPI00792186 ABCF1, ATP-binding cassette, sub-family F (GCN20), member 1 S106 T109 S110 2.73 NaN 1.20 NaN 
IPI00025512 HSPB1, Heat shock protein β-1 S78 S82  2.58 0.21 1.20 0.03 
IPI00438229 TRIM28, Isoform 1 of transcription intermediary factor 1-β S473   2.53 0.51 1.20 0.30 
IPI00102670 FNBP1, Isoform 2 of formin-binding protein 1 S296   2.42 0.20 0.77 0.13 
IPI00017338 PTPN7, Isoform 1 of tyrosine-protein phosphatase non-receptor S44   2.31 NaN 1.11 NaN 
IPI00025512 HSPB1, Heat shock protein β-1 S82   2.08 0.22 1.14 0.08 
IPI00302592 FLNA, Filamin A, α isoform 1 S2144   1.92 NaN 2.11 NaN 
IPI00217467 HIST1H1E, Histone H1.4 S36   1.90 0.05 3.10 0.02 
IPI00013835 DGKZ, Isoform 1 of diacylglycerol kinase ζ S37   0.86 NaN 0.40 NaN 
IPI00032970 OSBPL11, Oxysterol-binding protein-related protein 11 S189   0.09 NaN 0.02 NaN 
IPIProteinSite1Site2Site310 minutes
60 minutes
RatioSTDEVRatioSTDEV
IPI00289608 AP3D1, Isoform 2 of AP-3 complex subunit δ-1 S567   16.87 4.08 1.84 0.58 
IPI00418471 VIM, Vimentin S39   12.63 3.75 2.06 0.61 
IPI00102670 FNBP1, Isoform 2 of Formin-binding protein 1 S296 S301  11.79 1.47 1.13 0.04 
IPI00009334 PRKD2, Serine/threonine-protein kinase D2 S197 S198  10.43 NaN 2.37 NaN 
IPI00021389 CCS, Copper chaperone for superoxide dismutase S267   9.63 NaN 2.68 NaN 
IPI00004407 SIT1, Signaling threshold-regulating transmembrane adapter 1 S182   9.21 NaN 2.24 NaN 
IPI00791144 EPB41, EPB41 protein S549   8.66 NaN 2.52 NaN 
IPI00008994 NDRG2, Isoform 1 of protein NDRG2 S332   8.38 0.90 1.95 0.24 
IPI00418471 VIM, Vimentin S25   8.31 NaN 1.83 NaN 
IPI00292228 GSK3A, Glycogen synthase kinase-3 α S21   7.95 NaN 2.08 NaN 
IPI00789551 MATR3, Uncharacterized protein MATR3 T150   7.47 NaN 4.00 NaN 
IPI00410067 ZC3HAV1, Isoform 1 of zinc finger CCCH-type antiviral protein 1 S284   7.41 NaN 3.91 NaN 
IPI00410067 ZC3HAV1, Isoform 1 of zinc finger CCCH-type antiviral protein 1 S284   7.30 NaN 3.31 NaN 
IPI00014256 ARHGEF6, Isoform 1 of Rho guanine nucleotide exchange factor 6 S640   7.06 NaN 2.35 NaN 
IPI00027430 SPN, Leukosialin precursor T341   6.93 NaN 1.94 NaN 
IPI00007722 AMPD2, Adenosine monophosphate deaminase 2 S76   6.10 NaN 1.91 NaN 
IPI00301058 VASP, Vasodilator-stimulated phosphoprotein S239   6.03 0.39 1.84 0.18 
IPI00022449 DOCK2, Isoform 1 of dedicator of cytokinesis protein 2 S1706   5.78 NaN 1.63 NaN 
IPI00217393 CENTG1, Isoform 1 of centaurin-γ-1 S648   5.72 0.42 1.81 0.10 
IPI00026928 GRAP2, GRB2-related adapter protein 2 T262   5.29 1.87 2.61 0.83 
IPI00418471 VIM, Vimentin S73   4.54 1.28 2.68 0.90 
IPI00442053 ARHGAP9, Isoform 2 of Rho GTPase-activating protein 9 S481   4.47 0.24 1.61 0.09 
IPI00013174 RBM14, Isoform 1 of RNA-binding protein 14 S618   4.26 NaN 2.66 NaN 
IPI00028065 NCK1, Cytoplasmic protein NCK1 S85   4.24 NaN 1.85 NaN 
IPI00329495 ABLIM1, Isoform 1 of actin-binding LIM protein 1 S587   4.05 1.28 1.57 0.43 
IPI00785100 MEF2D, Myocyte enhancer factor 2D S121   4.01 NaN 1.86 NaN 
IPI00032355 PUM1, Pumilio homolog 1 S745   3.68 NaN 1.61 NaN 
IPI00171176 PANK2, Isoform 1 of pantothenate kinase 2, mitochondrial prec. S168   3.26 NaN 1.36 NaN 
IPI00297160 CD44, Isoform 12 of CD44 antigen precursor S316   2.90 NaN 2.66 NaN 
IPI00028027 GSDMDC1, Gasdermin domain-containing protein 1 S185   2.88 NaN 1.52 NaN 
IPI00418471 VIM, Vimentin S66   2.84 0.21 1.15 0.10 
IPI00792186 ABCF1, ATP-binding cassette, sub-family F (GCN20), member 1 S106 T109 S110 2.73 NaN 1.20 NaN 
IPI00025512 HSPB1, Heat shock protein β-1 S78 S82  2.58 0.21 1.20 0.03 
IPI00438229 TRIM28, Isoform 1 of transcription intermediary factor 1-β S473   2.53 0.51 1.20 0.30 
IPI00102670 FNBP1, Isoform 2 of formin-binding protein 1 S296   2.42 0.20 0.77 0.13 
IPI00017338 PTPN7, Isoform 1 of tyrosine-protein phosphatase non-receptor S44   2.31 NaN 1.11 NaN 
IPI00025512 HSPB1, Heat shock protein β-1 S82   2.08 0.22 1.14 0.08 
IPI00302592 FLNA, Filamin A, α isoform 1 S2144   1.92 NaN 2.11 NaN 
IPI00217467 HIST1H1E, Histone H1.4 S36   1.90 0.05 3.10 0.02 
IPI00013835 DGKZ, Isoform 1 of diacylglycerol kinase ζ S37   0.86 NaN 0.40 NaN 
IPI00032970 OSBPL11, Oxysterol-binding protein-related protein 11 S189   0.09 NaN 0.02 NaN 

Phosphopeptides predicted as possible PKA substrates by 2 or more kinase prediction algorithms or by in vitro experimentation. Ratios of change in phosphorylation are shown for both 10- and 60-minute time points together with the SD when applicable.

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