Gene expression data for Srf deletion
| . | Array data . | Quantitative RT-PCR . | ||
|---|---|---|---|---|
| Ratio*† . | P . | Ratio*‡ . | Ratio*† . | |
| Srf | 0.10 | < .001 | 0.04 | 0.05 |
| Coro1a | 0.18 | < .001 | 0.10 | 0.25 |
| Cnn2 | 0.32 | .009 | 0.07 | 0.33 |
| Flna | 0.35 | .009 | 0.15 | 0.47 |
| Myl6 | 0.57 | .324 | 0.56 | 0.57 |
| Mkl1 | 1.16 | .356 | ND | 1.75 |
| Mkl2 | NA | NA | 0.62 | 1.35 |
| . | Array data . | Quantitative RT-PCR . | ||
|---|---|---|---|---|
| Ratio*† . | P . | Ratio*‡ . | Ratio*† . | |
| Srf | 0.10 | < .001 | 0.04 | 0.05 |
| Coro1a | 0.18 | < .001 | 0.10 | 0.25 |
| Cnn2 | 0.32 | .009 | 0.07 | 0.33 |
| Flna | 0.35 | .009 | 0.15 | 0.47 |
| Myl6 | 0.57 | .324 | 0.56 | 0.57 |
| Mkl1 | 1.16 | .356 | ND | 1.75 |
| Mkl2 | NA | NA | 0.62 | 1.35 |
NA indicates not available in array; and ND, not done.
Ratio indicates mRNA expression ratio in KO/WT megakaryocyte.
Megakaryocyte progenitors were sorted by flow cytometry and cultured for 3 days in TPO-containing medium.
Whole BM was cultured for 4 days in TPO-containing medium and mature megakaryocytes were enriched by BSA gradient separation.