Patient and disease characteristics and TWIST levels in CD34+ cells
| Diagnosis . | Age, y . | Sex . | Cytogenetics . | IPSS . | BM cellularity . | Marrow blast count, % . | TWIST expression . | P53 expression . | TWIST/p53 ratio . |
|---|---|---|---|---|---|---|---|---|---|
| RA | 59 | F | Normal | 0 | Normal | 3 | 1.1739385 | 0.9980367 | 1.176248 |
| RA | 58 | F | Normal | 0 | Normal | 5 | 1.1937184 | 0.9743392 | 1.225157 |
| RA | 51 | M | Normal | 0.5 | Hypo | 3 | 1.2863391 | 0.9798507 | 1.312791 |
| RA | 61 | F | Normal | NA | Normal | 3 | 1.1456094 | 0.9826767 | 1.165805 |
| RA | 78 | M | Normal | 0.5 | Normal | 2 | 1.1686 | 0.9732649 | 1.200706 |
| RA | 79 | M | Normal | 0.5 | Hypo | 5 | 0.8357093 | 0.9982278 | 0.837193 |
| RA | 58 | M | Normal | 0 | Normal | 4 | 1.2723986 | 1.0561759 | 1.204722 |
| RA | 46 | F | Normal | 0 | Hyper | 3 | 1.0282371 | 1.1760294 | 0.874329 |
| RA | 45 | F | Normal | NA | Hyper | 5 | 1.1807 | 1.1183639 | 1.055771 |
| RA | 58 | M | Del 7 | NA | Hyper | 4 | 1.3967883 | 0.9036251 | 1.545761 |
| RA | 58 | F | Del 5 | 0 | Normal | 5 | 0.9442959 | 0.7455998 | 1.266492 |
| RA | 59 | F | Normal | 0 | Hyper | 5 | 1.0647 | 0.8619087 | 1.235282 |
| RA | 56 | F | Normal | NA | Hyper | 3 | 1.0298 | 0.7042588 | 1.462247 |
| RA | 57 | M | Tri 9, tri 11 | 1 | Normal | 3 | 1.1586889 | 0.9124743 | 1.269832 |
| RA | 76 | M | Tri 5, tri 9, del 6 | 0.5 | Hyper | 1.5 | 1.2326 | 1.079136 | 1.142203 |
| RA | 56 | F | Normal | NA | Normal | 5 | 1.2120478 | 0.9923114 | 1.221439 |
| RA | 65 | F | Normal | NA | Hyper | 5 | 1.1739385 | 0.9980367 | 1.176248 |
| RA | 82 | M | Normal | 0 | Hyper | 4 | 1.5250296 | 1.0450261 | 1.459322 |
| RA | 65 | M | Complex | 0 | Hyper | 5 | 1.0842018 | 0.7963254 | 1.361506 |
| RA | 69 | F | Normal | 1 | Hyper | 2 | 0.7038678 | 0.852639 | 0.825517 |
| RA | 82 | M | Normal | 0 | Hyper | 5 | 0.7189514 | 0.623189 | 1.153665 |
| RA | 78 | M | Normal | 0 | Hyper | 3 | 1.7513135 | 0.9201176 | 1.903358 |
| RA | 43 | F | Tri 1 | 1.0 | Hyper | 5 | 1.1859731 | 0.8560692 | 1.385371 |
| RA | 60 | F | Tri 15 11q− | NA | Hyper | 2 | 1.1355641 | 0.84988 | 1.336146 |
| RARS | 48 | F | t(3,21) | 0 | Hypo | 5 | 1.3050591 | 0.9460929 | 1.37942 |
| RARS | 76 | F | Normal | 0 | Hyper | 5 | 1.2224846 | 0.8011956 | 1.525826 |
| RARS | 40 | F | Del(5q) tri8 | NA | Hypo | 5 | 1.1226 | 1.1519293 | 0.974581 |
| RARS | 48 | M | Tri 8 | 0.5 | Normal | 5 | 0.4877078 | 0.4587859 | 1.06304 |
| RCMD | 50 | F | Mono X | 0.5 | Hyper | 5 | 1.1723329 | 0.8742435 | 1.340968 |
| RCMD | 49 | F | Complex | 1.5 | Hyper | 5 | 1.1680894 | 1.533244 | 0.761842 |
| RCMD | 65 | F | Complex | 1.5 | Normal | 3 | 1.1355641 | 0.84988 | 1.336146 |
| RCMD | 60 | M | Normal | 0.5 | Normal | 5 | 1.3050591 | 0.9460929 | 1.37942 |
| RCMD | 54 | M | Del 7 | 1.5 | Hyper | 5 | 1.565455 | 0.9456547 | 1.656546 |
| RAEB-1 | 59 | M | Normal | 0.5 | Normal | 8 | 1.2224846 | 0.8011956 | 1.525826 |
| RAEB-1 | 71 | M | Complex, del(7) | 1.5 | Normal | 7 | 1.1226 | 1.1519293 | 0.974581 |
| RAEB-1 | 78 | M | Tri8 | 2.5 | Hyper | 6 | 1.0843432 | 0.881764 | 1.229743 |
| RAEB-1 | 71 | F | Mono 7 | 1.5 | Hypo | 6 | 1.2456 | 1.0451892 | 1.19171 |
| RAEB-1 | 52 | F | 11q− | 1.5 | Hyper | 7 | 1.8943619 | 1.1587836 | 1.634785 |
| RAEB-1 | 52 | M | +8, +21 | 0.5 | Hyper | 8 | 1.1292417 | 0.7932933 | 1.423486 |
| RAEB-2 | 50 | M | Normal | 2 | Hyper | 12 | 1.5372 | 1.0765506 | 1.427912 |
| RAEB-2 | 58 | M | Normal | 0.0 | Normal | 15 | 1.1358 | 0.9012022 | 1.260262 |
| RAEB-2 | 61 | M | y− | 2.5 | Hyper | 16 | 1.4958 | 0.686075 | 2.180228 |
| RAEB-2 | 48 | F | Complex | 2.0 | Normal | 19 | 1.1358 | 0.9012022 | 1.260262 |
| RAEB-2 | 56 | M | Del 7 | 2 | Normal | 12 | 1.0385472 | 1.0036802 | 1.034739 |
| RAEB-2 | 68 | M | Complex | 1.5 | Normal | 10 | 1.2003644 | 0.6438533 | 1.864345 |
| RAEB-2 | 76 | F | Complex | 2 | Hyper | 13 | 1.1573 | 0.9615516 | 1.203537 |
| RAEB-2 | 57 | M | Normal | 1.5 | Normal | 15 | 1.2841701 | 0.9245436 | 1.388977 |
| RAEB-2 | 63 | F | Complex | 1.0 | Hyper | 11 | 1.0836589 | 0.8363269 | 1.295736 |
| MDS sec | 65 | M | Normal | Hyper | 2 | 1.0765132 | 0.7689531 | 1.399972 | |
| MDS sec | 58 | M | Normal | Hyper | 5 | 1.2723986 | 1.0561759 | 1.204722 | |
| MDS sec | 57 | M | Complex | Hyper | 10 | 1.670892 | 1.0202264 | 1.637766 | |
| MDS sec | 55 | F | Normal | Normal | 7 | 1.1392 | 0.8290421 | 1.146339 | |
| MDS sec | 48 | M | Complex | Hyper | 11 | 1.390892 | 1.8638578 | 0.746244 | |
| MDS sec | 63 | F | Complex | Hyper | 5 | 1.1392 | 0.8290421 | 1.146339 | |
| MDS sec | 58 | F | Der (5)t(5;17)(p10;q11), −17/idem, t(1;10) | Hyper | 7 | 1.9175455 | 0.8482369 | 2.260625 | |
| MDS sec | 57 | M | Complex | Normal | 12 | 1.3744509 | 0.8745623 | 1.571587 |
| Diagnosis . | Age, y . | Sex . | Cytogenetics . | IPSS . | BM cellularity . | Marrow blast count, % . | TWIST expression . | P53 expression . | TWIST/p53 ratio . |
|---|---|---|---|---|---|---|---|---|---|
| RA | 59 | F | Normal | 0 | Normal | 3 | 1.1739385 | 0.9980367 | 1.176248 |
| RA | 58 | F | Normal | 0 | Normal | 5 | 1.1937184 | 0.9743392 | 1.225157 |
| RA | 51 | M | Normal | 0.5 | Hypo | 3 | 1.2863391 | 0.9798507 | 1.312791 |
| RA | 61 | F | Normal | NA | Normal | 3 | 1.1456094 | 0.9826767 | 1.165805 |
| RA | 78 | M | Normal | 0.5 | Normal | 2 | 1.1686 | 0.9732649 | 1.200706 |
| RA | 79 | M | Normal | 0.5 | Hypo | 5 | 0.8357093 | 0.9982278 | 0.837193 |
| RA | 58 | M | Normal | 0 | Normal | 4 | 1.2723986 | 1.0561759 | 1.204722 |
| RA | 46 | F | Normal | 0 | Hyper | 3 | 1.0282371 | 1.1760294 | 0.874329 |
| RA | 45 | F | Normal | NA | Hyper | 5 | 1.1807 | 1.1183639 | 1.055771 |
| RA | 58 | M | Del 7 | NA | Hyper | 4 | 1.3967883 | 0.9036251 | 1.545761 |
| RA | 58 | F | Del 5 | 0 | Normal | 5 | 0.9442959 | 0.7455998 | 1.266492 |
| RA | 59 | F | Normal | 0 | Hyper | 5 | 1.0647 | 0.8619087 | 1.235282 |
| RA | 56 | F | Normal | NA | Hyper | 3 | 1.0298 | 0.7042588 | 1.462247 |
| RA | 57 | M | Tri 9, tri 11 | 1 | Normal | 3 | 1.1586889 | 0.9124743 | 1.269832 |
| RA | 76 | M | Tri 5, tri 9, del 6 | 0.5 | Hyper | 1.5 | 1.2326 | 1.079136 | 1.142203 |
| RA | 56 | F | Normal | NA | Normal | 5 | 1.2120478 | 0.9923114 | 1.221439 |
| RA | 65 | F | Normal | NA | Hyper | 5 | 1.1739385 | 0.9980367 | 1.176248 |
| RA | 82 | M | Normal | 0 | Hyper | 4 | 1.5250296 | 1.0450261 | 1.459322 |
| RA | 65 | M | Complex | 0 | Hyper | 5 | 1.0842018 | 0.7963254 | 1.361506 |
| RA | 69 | F | Normal | 1 | Hyper | 2 | 0.7038678 | 0.852639 | 0.825517 |
| RA | 82 | M | Normal | 0 | Hyper | 5 | 0.7189514 | 0.623189 | 1.153665 |
| RA | 78 | M | Normal | 0 | Hyper | 3 | 1.7513135 | 0.9201176 | 1.903358 |
| RA | 43 | F | Tri 1 | 1.0 | Hyper | 5 | 1.1859731 | 0.8560692 | 1.385371 |
| RA | 60 | F | Tri 15 11q− | NA | Hyper | 2 | 1.1355641 | 0.84988 | 1.336146 |
| RARS | 48 | F | t(3,21) | 0 | Hypo | 5 | 1.3050591 | 0.9460929 | 1.37942 |
| RARS | 76 | F | Normal | 0 | Hyper | 5 | 1.2224846 | 0.8011956 | 1.525826 |
| RARS | 40 | F | Del(5q) tri8 | NA | Hypo | 5 | 1.1226 | 1.1519293 | 0.974581 |
| RARS | 48 | M | Tri 8 | 0.5 | Normal | 5 | 0.4877078 | 0.4587859 | 1.06304 |
| RCMD | 50 | F | Mono X | 0.5 | Hyper | 5 | 1.1723329 | 0.8742435 | 1.340968 |
| RCMD | 49 | F | Complex | 1.5 | Hyper | 5 | 1.1680894 | 1.533244 | 0.761842 |
| RCMD | 65 | F | Complex | 1.5 | Normal | 3 | 1.1355641 | 0.84988 | 1.336146 |
| RCMD | 60 | M | Normal | 0.5 | Normal | 5 | 1.3050591 | 0.9460929 | 1.37942 |
| RCMD | 54 | M | Del 7 | 1.5 | Hyper | 5 | 1.565455 | 0.9456547 | 1.656546 |
| RAEB-1 | 59 | M | Normal | 0.5 | Normal | 8 | 1.2224846 | 0.8011956 | 1.525826 |
| RAEB-1 | 71 | M | Complex, del(7) | 1.5 | Normal | 7 | 1.1226 | 1.1519293 | 0.974581 |
| RAEB-1 | 78 | M | Tri8 | 2.5 | Hyper | 6 | 1.0843432 | 0.881764 | 1.229743 |
| RAEB-1 | 71 | F | Mono 7 | 1.5 | Hypo | 6 | 1.2456 | 1.0451892 | 1.19171 |
| RAEB-1 | 52 | F | 11q− | 1.5 | Hyper | 7 | 1.8943619 | 1.1587836 | 1.634785 |
| RAEB-1 | 52 | M | +8, +21 | 0.5 | Hyper | 8 | 1.1292417 | 0.7932933 | 1.423486 |
| RAEB-2 | 50 | M | Normal | 2 | Hyper | 12 | 1.5372 | 1.0765506 | 1.427912 |
| RAEB-2 | 58 | M | Normal | 0.0 | Normal | 15 | 1.1358 | 0.9012022 | 1.260262 |
| RAEB-2 | 61 | M | y− | 2.5 | Hyper | 16 | 1.4958 | 0.686075 | 2.180228 |
| RAEB-2 | 48 | F | Complex | 2.0 | Normal | 19 | 1.1358 | 0.9012022 | 1.260262 |
| RAEB-2 | 56 | M | Del 7 | 2 | Normal | 12 | 1.0385472 | 1.0036802 | 1.034739 |
| RAEB-2 | 68 | M | Complex | 1.5 | Normal | 10 | 1.2003644 | 0.6438533 | 1.864345 |
| RAEB-2 | 76 | F | Complex | 2 | Hyper | 13 | 1.1573 | 0.9615516 | 1.203537 |
| RAEB-2 | 57 | M | Normal | 1.5 | Normal | 15 | 1.2841701 | 0.9245436 | 1.388977 |
| RAEB-2 | 63 | F | Complex | 1.0 | Hyper | 11 | 1.0836589 | 0.8363269 | 1.295736 |
| MDS sec | 65 | M | Normal | Hyper | 2 | 1.0765132 | 0.7689531 | 1.399972 | |
| MDS sec | 58 | M | Normal | Hyper | 5 | 1.2723986 | 1.0561759 | 1.204722 | |
| MDS sec | 57 | M | Complex | Hyper | 10 | 1.670892 | 1.0202264 | 1.637766 | |
| MDS sec | 55 | F | Normal | Normal | 7 | 1.1392 | 0.8290421 | 1.146339 | |
| MDS sec | 48 | M | Complex | Hyper | 11 | 1.390892 | 1.8638578 | 0.746244 | |
| MDS sec | 63 | F | Complex | Hyper | 5 | 1.1392 | 0.8290421 | 1.146339 | |
| MDS sec | 58 | F | Der (5)t(5;17)(p10;q11), −17/idem, t(1;10) | Hyper | 7 | 1.9175455 | 0.8482369 | 2.260625 | |
| MDS sec | 57 | M | Complex | Normal | 12 | 1.3744509 | 0.8745623 | 1.571587 |
IPSS indicates International Prognostic Scoring System; RA, refractory anemia; normal, normal karyotype; hypo, cellularity lower than expected for age; hyper, cellularity greater than expected for age; NA, not available because of incomplete information on peripheral blood cell counts or no scores were assigned to secondary MDS; RARS, RA with ring sideroblasts; RCMD, refractory cytopenia with multilineage dysplasia; RAEB, RA with excess blasts (1 or 2); and MDS sec, secondary (treatment-related) myelodysplastic syndrome.