Ig synthesis in IgH 3′RR-deficient mice
| . | Ig isotype . | Wild-type mice . | IgH 3′RR-deficient mice . | P . |
|---|---|---|---|---|
| Splenocyte supernatant, ng/mL* | ||||
| LPS | IgM | 21 333.3 ± 8855.5 | 1652.5 ± 685.0 | .009 |
| LPS | IgG3 | 445.3 ± 259.2 | 0 ± 0 | < .001 |
| LPS | IgG2b | 155.4 ± 71.8 | 4.8 ± 2 | .001 |
| LPS + IL-4 | IgG1 | 2402.5 ± 1040.6 | 13.5 ± 0.7 | < .001 |
| LPS + IL-4 | IgE | 14.6 ± 6.4 | 0 ± 0 | < .001 |
| LPS + IFN-γ | IgG2a | 4.8 ± 2.8 | 0 ± 0 | .001 |
| LPS + TGF-β | IgA | 353.5 ± 191.2 | 0 ± 0 | < .001 |
| Serum, μg/mL† | ||||
| IgM | 283.5 ± 52.4 | 23.7 ± 2.4 | < .001 | |
| IgG3 | 9.7 ± 1.9 | 0 ± 0 | < .001 | |
| IgG1 | 59.2 ± 18.4 | 37.7 ± 9.5 | .45 | |
| IgG2b | 1.9 ± 0.1 | 0.9 ± 0.1 | < .001 | |
| IgG2a | 63.2 ± 32.8 | 82.1 ± 19.0 | .41 | |
| IgE | 2.3 ± 0.7 | 0 ± 0 | < .001 | |
| IgA | 33.6 ± 5.8 | 2.9 ± 0.7 | < .001 |
| . | Ig isotype . | Wild-type mice . | IgH 3′RR-deficient mice . | P . |
|---|---|---|---|---|
| Splenocyte supernatant, ng/mL* | ||||
| LPS | IgM | 21 333.3 ± 8855.5 | 1652.5 ± 685.0 | .009 |
| LPS | IgG3 | 445.3 ± 259.2 | 0 ± 0 | < .001 |
| LPS | IgG2b | 155.4 ± 71.8 | 4.8 ± 2 | .001 |
| LPS + IL-4 | IgG1 | 2402.5 ± 1040.6 | 13.5 ± 0.7 | < .001 |
| LPS + IL-4 | IgE | 14.6 ± 6.4 | 0 ± 0 | < .001 |
| LPS + IFN-γ | IgG2a | 4.8 ± 2.8 | 0 ± 0 | .001 |
| LPS + TGF-β | IgA | 353.5 ± 191.2 | 0 ± 0 | < .001 |
| Serum, μg/mL† | ||||
| IgM | 283.5 ± 52.4 | 23.7 ± 2.4 | < .001 | |
| IgG3 | 9.7 ± 1.9 | 0 ± 0 | < .001 | |
| IgG1 | 59.2 ± 18.4 | 37.7 ± 9.5 | .45 | |
| IgG2b | 1.9 ± 0.1 | 0.9 ± 0.1 | < .001 | |
| IgG2a | 63.2 ± 32.8 | 82.1 ± 19.0 | .41 | |
| IgE | 2.3 ± 0.7 | 0 ± 0 | < .001 | |
| IgA | 33.6 ± 5.8 | 2.9 ± 0.7 | < .001 |
ELISA analysis of IgM, IgG3, IgG1, IgG2b, IgG2a, IgE, and IgA in supernatants of B cells cultured for 4 days with LPS with or without cytokines. Data are mean ± SEM of 9 independent experiments (Mann-Whitney U test for significance).
ELISA analysis of IgM, IgG3, IgG1, IgG2b, IgG2a, IgE, and IgA in 8-week-old mouse sera of wt and mutant mice. Data are mean ± SEM of 7 animals in each group (Mann-Whitney U test for significance).