Genes of interest from literature
| Gene . | Gene full name . | Chromosome . | Rationale for selection . |
|---|---|---|---|
| FHIT | Fragile histidine triad | Chr 3p14.2 | Known to be hypermethylated in MLL-r infant ALL41 |
| FLT3 | FMS-like tyrosine kinase 3 | Chr 13q12.2 | Known to be up-regulated and of biologic importance in MLL-r infant ALL39 |
| HOXa9 | Homeobox A9 | Chr 7p15-p14 | Known to be up-regulated and of biologic importance in MLL-r infant ALL11 |
| MEIS1 | MEIS-homeobox 1 | Chr 2p14-p13 | Known to be up-regulated and of biologic importance in MLL-r infant ALL11 |
| GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | Chr 12p13.31 | Housekeeping gene |
| ABL | C-ABL oncogene, receptor tyrosine kinase | Chr 9q34.1 | Housekeeping gene |
| Gene . | Gene full name . | Chromosome . | Rationale for selection . |
|---|---|---|---|
| FHIT | Fragile histidine triad | Chr 3p14.2 | Known to be hypermethylated in MLL-r infant ALL41 |
| FLT3 | FMS-like tyrosine kinase 3 | Chr 13q12.2 | Known to be up-regulated and of biologic importance in MLL-r infant ALL39 |
| HOXa9 | Homeobox A9 | Chr 7p15-p14 | Known to be up-regulated and of biologic importance in MLL-r infant ALL11 |
| MEIS1 | MEIS-homeobox 1 | Chr 2p14-p13 | Known to be up-regulated and of biologic importance in MLL-r infant ALL11 |
| GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | Chr 12p13.31 | Housekeeping gene |
| ABL | C-ABL oncogene, receptor tyrosine kinase | Chr 9q34.1 | Housekeeping gene |
To complete the list of genes for qRT-PCR analysis from Table 2, 6 additional genes were selected. One gene, FHIT, was selected based on known reports of it being hypermethylated in MLL-r infant leukemia; 3 genes (FLT3, HOXa9, and MEIS1) were selected based on their known importance in MLL-r infant leukemia; and 2 housekeeping genes, ABL and GAPDH, were also selected.