Table 1

Angioblast phenotypes in ECSCR loss of function

InjectionPhenotype, % of observed
NormalMildSevereToxic
cMO 14 ng (36) 83 11 
MO1 10 ng (20)* 31 54 15 
MO2 10 ng (18)* 22 61 17 
MO2 14 ng (17) 29 24 24 24 
MO2 10 ng + hECSCR mRNA (37) 62 30 
InjectionPhenotype, % of observed
NormalMildSevereToxic
cMO 14 ng (36) 83 11 
MO1 10 ng (20)* 31 54 15 
MO2 10 ng (18)* 22 61 17 
MO2 14 ng (17) 29 24 24 24 
MO2 10 ng + hECSCR mRNA (37) 62 30 

Zebrafish were injected with morpholinos as indicated, and scored at 14 som for etsrp+ angioblast positioning. Numbers in parentheses indicate the total number scored, and the incidence of each possible phenotype is given as a percentage of all scored embryos. Criteria: Embryos were scored as normal if there was evidence of a nascent midline vascular cord; mildly affected if midline angioblasts were sparse or absent; severely affected if individual angioblasts appeared lateral to the main row (ie, wrong direction); and toxic if the entire embryo appeared to be adversely affected.

ECSCR indicates endothelial-cell specific chemotaxis receptor.

Significant differences of observed distributions are indicated as follows: *P < .001 versus cMO injected;

P < .001 versus MO2 10 ng injected, using χ2 test excluding embryos scored as toxic.

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