Primers used in this study
| Primer . | Direction . | Sequence . |
|---|---|---|
| TLR9 | Forward | 5′-ACC CTG GAA GAG CTA AAC C-3′ |
| Reverse | 5′-CAG TTG CCG TCC ATG AAT-3′ | |
| EPOR | Forward | 5′-TCA TCC TCG TGG TCA TCC T-3′ |
| Reverse | 5′-CCT TCA AAC TCG CTC TCT G-3′ | |
| GATA-1 | Forward | 5′-TGG CCT ACT ACA GGG ACG CT-3′ |
| Reverse | 5′-CTC AGC CGC TCT GTC TTC A-3′ | |
| GATA-2 | Forward | 5′-CCT CCA GCT TCA CCC CTA A-3′ |
| Reverse | 5′-CAC AGG CAT TGC ACA GGT AGT-3′ | |
| EKLF | Forward | 5′-CAG AGG ATC CAG GTG TGA TAG-3′ |
| Reverse | 5′-GCA GGC GTA TGG CTT CTC-3′ | |
| RPS19 | Forward | 5′-GCT CCA TGA CCA AGA TCT AT-3′ |
| Reverse | 5′-GTC CAG ATC TCT TTG TCC CT-3′ | |
| FOG-1 | Forward | 5′-TGC ACA CGG ACA CGC TGA-3′ |
| Reverse | 5′-GTA GAT CTC ACC CTT GGA GCC A-3′ | |
| 28S | Forward | 5′-TGG GTT TTA AGC AGG AGG TG-3′ |
| Reverse | 5′-CCA GCT CAC GTT CCC TAT TA-3′ |
| Primer . | Direction . | Sequence . |
|---|---|---|
| TLR9 | Forward | 5′-ACC CTG GAA GAG CTA AAC C-3′ |
| Reverse | 5′-CAG TTG CCG TCC ATG AAT-3′ | |
| EPOR | Forward | 5′-TCA TCC TCG TGG TCA TCC T-3′ |
| Reverse | 5′-CCT TCA AAC TCG CTC TCT G-3′ | |
| GATA-1 | Forward | 5′-TGG CCT ACT ACA GGG ACG CT-3′ |
| Reverse | 5′-CTC AGC CGC TCT GTC TTC A-3′ | |
| GATA-2 | Forward | 5′-CCT CCA GCT TCA CCC CTA A-3′ |
| Reverse | 5′-CAC AGG CAT TGC ACA GGT AGT-3′ | |
| EKLF | Forward | 5′-CAG AGG ATC CAG GTG TGA TAG-3′ |
| Reverse | 5′-GCA GGC GTA TGG CTT CTC-3′ | |
| RPS19 | Forward | 5′-GCT CCA TGA CCA AGA TCT AT-3′ |
| Reverse | 5′-GTC CAG ATC TCT TTG TCC CT-3′ | |
| FOG-1 | Forward | 5′-TGC ACA CGG ACA CGC TGA-3′ |
| Reverse | 5′-GTA GAT CTC ACC CTT GGA GCC A-3′ | |
| 28S | Forward | 5′-TGG GTT TTA AGC AGG AGG TG-3′ |
| Reverse | 5′-CCA GCT CAC GTT CCC TAT TA-3′ |
TLR9 indicates Toll-like receptor 9; EPOR, erythropoietin receptor; EKLF, erythroid Krüppel-like factor; RPS19, ribosomal protein S19; FOG-1, friend of GATA-1; and 28S, 28S ribosomal gene.