The concentration of protamine and RNA affects particle size
| . | Particle size in nm . | |||
|---|---|---|---|---|
| 2 mg/mL . | 1 mg/mL . | 0.5 mg/mL . | 0.25 mg/mL . | |
| Water | 575 | 267 | 274 | 263 |
| 25mM NaCl | 492 | 277 | ||
| 75mM NaCl | 1045 | 512 | ||
| 225mM NaCl | 1288 | 869 | ||
| Ringer lactate | 1363 | 1432 | 926 | 571 |
| . | Particle size in nm . | |||
|---|---|---|---|---|
| 2 mg/mL . | 1 mg/mL . | 0.5 mg/mL . | 0.25 mg/mL . | |
| Water | 575 | 267 | 274 | 263 |
| 25mM NaCl | 492 | 277 | ||
| 75mM NaCl | 1045 | 512 | ||
| 225mM NaCl | 1288 | 869 | ||
| Ringer lactate | 1363 | 1432 | 926 | 571 |
Protamine 5000 was diluted 7 times (∼ 2 mg/mL final), 14 times (∼ 1 mg/mL final), 28 times (∼ 0.5 mg/mL final), or 56 times (∼0.25 mg/mL final) with water, 25mM NaCl, 75mM NaCl, 225mM NaCl, or ringer lactate. Similarly, the RNA oligonucleotide RNA18 was diluted to the same final concentrations as the above-mentioned 5 solutions. Protamine and RNA were mixed at a 1:1 mass ratio. Subsequently, the size of the particle was measured by dynamic light scattering.