Table 1 Self-association, Ras binding,... | American Society of Hematology

Table 1

Self-association, Ras binding, and oncogenic potentials of the AF6 RA1 and RA2 domains

MLL construct	Transformation	Ras binding, fold reduction*	Ras association by MLL-AF6†	Self-association
RA1 + RA2	+	1	ND	+
RA1	+	1	+	+
RA1 K58A	+	7.1	−	+
RA1 K78A	+	6.2	−	+
RA1 ΔN	−	ND	−	−
RA1 ΔC	−	ND	−	−
L + RA2	−	10.6	−	−

MLL construct	Transformation	Ras binding, fold reduction*	Ras association by MLL-AF6†	Self-association
RA1 + RA2	+	1	ND	+
RA1	+	1	+	+
RA1 K58A	+	7.1	−	+
RA1 K78A	+	6.2	−	+
RA1 ΔN	−	ND	−	−
RA1 ΔC	−	ND	−	−
L + RA2	−	10.6	−	−

ND indicates not determined.

Data from Wohlgemuth et al.⁴⁰

Determined by coimmunoprecipitation with MLL-AF6 constructs (Figure 2D).