Effect of storage with or without p38 MAPK inhibitor on platelet parameters
| . | Fresh PRP . | Stored PRP (vehicle) . | Stored PRP/SB203580 . |
|---|---|---|---|
| P-selectin, mean (± SEM) | 4.3 (± 0.1) | 12.9 (± 2.2)** | 6.3 (± 0.4) |
| GPIV, mean (± SEM) | 20.3 (± 0.5) | 17.6 (± 2.0) | 19.0 (± 0.8) |
| CD9, mean (± SEM) | 1133 (± 35.6) | 1034 (± 123.8) | 1024 (± 45.0) |
| Phosphatidylserine, mean (± SEM) | 9.3 (± 0.3) | 40.8 (± 7.9)*** | 28.2 (± 3.8)* |
| Microparticles, mean (± SEM) | 29.4 (± 2.9) | 201.0 (± 16.5)*** | 169.6 (± 12.0)*** |
| . | Fresh PRP . | Stored PRP (vehicle) . | Stored PRP/SB203580 . |
|---|---|---|---|
| P-selectin, mean (± SEM) | 4.3 (± 0.1) | 12.9 (± 2.2)** | 6.3 (± 0.4) |
| GPIV, mean (± SEM) | 20.3 (± 0.5) | 17.6 (± 2.0) | 19.0 (± 0.8) |
| CD9, mean (± SEM) | 1133 (± 35.6) | 1034 (± 123.8) | 1024 (± 45.0) |
| Phosphatidylserine, mean (± SEM) | 9.3 (± 0.3) | 40.8 (± 7.9)*** | 28.2 (± 3.8)* |
| Microparticles, mean (± SEM) | 29.4 (± 2.9) | 201.0 (± 16.5)*** | 169.6 (± 12.0)*** |
Surface expressions of P-selectin, GPVI, CD9, phosphatidylserine and microparticle counts were determined by flow cytometry. Microparticles are defined as particles smaller than platelets that are double positive for GPIX and GPIIb. Results are shown as mean ± SEM. Microparticles released per 1000 platelets, n = 6.
MAPK indicates p38 mitogen-activated kinase; and PRP, platelet-rich plasma.
Statistically different to Fresh PRP at p < 0.001
Statistically different to Fresh PRP at p < 0.01
Statistically different to Fresh PRP at p < 0.05