Effect of pharmacologic inhibitors on GPIb-α shedding during platelet storage
| Mouse platelets . | GPIb-α mean fluorescence (± SEM) . | P from stored PRP . |
|---|---|---|
| Fresh PRPr | 421.9 (± 12.6) | < 0.001 |
| Stored PRP (Vehicle) | 34.1 (± 5.5) | |
| Stored PRP/PKC inhibitor (Ro 31-8220 2 μg/mL) | 28.5 (± 10.9) | n.s. |
| Stored PRP/ERK inhibitor (PD98059 20μM) | 28.2 (± 8.9) | n.s. |
| Stored PRP/Caspases inhibitor (Z-VAD 20μM) | 38.7 (± 9.1) | n.s. |
| Mouse platelets . | GPIb-α mean fluorescence (± SEM) . | P from stored PRP . |
|---|---|---|
| Fresh PRPr | 421.9 (± 12.6) | < 0.001 |
| Stored PRP (Vehicle) | 34.1 (± 5.5) | |
| Stored PRP/PKC inhibitor (Ro 31-8220 2 μg/mL) | 28.5 (± 10.9) | n.s. |
| Stored PRP/ERK inhibitor (PD98059 20μM) | 28.2 (± 8.9) | n.s. |
| Stored PRP/Caspases inhibitor (Z-VAD 20μM) | 38.7 (± 9.1) | n.s. |
Surface expression of GPIbα was measured by flow cytometry on fresh platelets or platelets stored in presence of inhibitors of PKC (Ro 31-8220), ERK mitogen-activated protein kinase (PD98059), caspases (Z-VAD), or dimethyl sulfoxide (Vehicle). Results are given as mean ± SEM, n = 8.