Donor and recipient HLA types and donor-matched EBV epitopes for patients 1 through 3
| Patient . | HLA-A . | HLA-B . | HLA-DRB1 . | Class I peptides matched to the donor . | Class II peptides matched to the donor . |
|---|---|---|---|---|---|
| Donor 1 | *0201 | *1501, *0702 | *1301, *1501 | CLG,†GLC,†YVL,† RPP† | FGQL, IPQC, TDAW |
| Patient 1 | *01, *02 | *1501, *51 | *1301, *0401 | ||
| Donor 2 | *0201 | *2702, *4402 | *0301, *1601 | CLG,†GLC,†YVL,†RRIY,†VEI,†‡KEH | None |
| Patient 2 | *0201 | *2702, *4402 | *0301, *1601 | ||
| Donor 3 | 1, 29 | 44, 51 | *1501, *07xx | VEI,†KEH | TDAW† |
| Patient 3 | 28, 29 | 44, 51 | *1501, *1101 | ||
| Donor 4 | *0101, *2402 | *0702, *3701 | *11, *15 | RYS, TYS, TYP | TDAW |
| Patient 4 | *01, *02 | *37, *55 | *03, *11 | ||
| Donor 5 | *0301, *2501 | *1501, *1801 | *0101, *0401 | RLR,† DEV | PYYV |
| Patient 5 | *0301, *2501 | *1501, *1518 | *0401, *0404 | ||
| Donor 6 | *2301, *2601 | *2705, *3801 | *0301, *1202 | RRIY | |
| Patient 6 | *0301, *2301 | *0702, *3801 | *0301, *0701 |
| Patient . | HLA-A . | HLA-B . | HLA-DRB1 . | Class I peptides matched to the donor . | Class II peptides matched to the donor . |
|---|---|---|---|---|---|
| Donor 1 | *0201 | *1501, *0702 | *1301, *1501 | CLG,†GLC,†YVL,† RPP† | FGQL, IPQC, TDAW |
| Patient 1 | *01, *02 | *1501, *51 | *1301, *0401 | ||
| Donor 2 | *0201 | *2702, *4402 | *0301, *1601 | CLG,†GLC,†YVL,†RRIY,†VEI,†‡KEH | None |
| Patient 2 | *0201 | *2702, *4402 | *0301, *1601 | ||
| Donor 3 | 1, 29 | 44, 51 | *1501, *07xx | VEI,†KEH | TDAW† |
| Patient 3 | 28, 29 | 44, 51 | *1501, *1101 | ||
| Donor 4 | *0101, *2402 | *0702, *3701 | *11, *15 | RYS, TYS, TYP | TDAW |
| Patient 4 | *01, *02 | *37, *55 | *03, *11 | ||
| Donor 5 | *0301, *2501 | *1501, *1801 | *0101, *0401 | RLR,† DEV | PYYV |
| Patient 5 | *0301, *2501 | *1501, *1518 | *0401, *0404 | ||
| Donor 6 | *2301, *2601 | *2705, *3801 | *0301, *1202 | RRIY | |
| Patient 6 | *0301, *2301 | *0702, *3801 | *0301, *0701 |
Only EBV peptide epitopes that are included in the peptide pool used for T-cell isolation (Table 1) were tested and are represented in the table. HLA allotypes shared by donor and patient, and epitopes restricted through these allotypes, are printed in boldface.
Epitopes that were recognized by antigen-specific T cells in the patients after T-cell transfer. An explanation of epitope designations and HLA restrictions is found in Table 1.
Responses against this epitope in patient 2 were seen in ELISPOT assays (data not shown).