Primers used for quantitative PCR
| Human mRNA . | Forward primer . | Reverse primer . | Accession no. . |
|---|---|---|---|
| TLR2 | GAGCCACAAAACTGTCTTTGTTGC | AACCTAGGACTTTATCCCAGCTCTC | NM_003263 |
| TLR4 | ACTGCAGGTGCTAGATTTATCCAG | GTCCAATGGGGAAGTTGTCTAGAG | XM_057452 |
| TLR5 | TATCAGGACAGTCACAGCTTCATC | AATACAGCATCAGAGAGACCACAG | NM_003268 |
| TLR8 | CACTTCAGTGTTAGGGAACATCAG | TTTCTTCGGCGCATAACTCACAGG | NM_016610 |
| CXCL8 (IL-8) | CTCTTGGCAGCCTTCCTGATTTCT | CAAGGAAAACTGGGTGCAGAGGGT | NM_000584 |
| Human mRNA . | Forward primer . | Reverse primer . | Accession no. . |
|---|---|---|---|
| TLR2 | GAGCCACAAAACTGTCTTTGTTGC | AACCTAGGACTTTATCCCAGCTCTC | NM_003263 |
| TLR4 | ACTGCAGGTGCTAGATTTATCCAG | GTCCAATGGGGAAGTTGTCTAGAG | XM_057452 |
| TLR5 | TATCAGGACAGTCACAGCTTCATC | AATACAGCATCAGAGAGACCACAG | NM_003268 |
| TLR8 | CACTTCAGTGTTAGGGAACATCAG | TTTCTTCGGCGCATAACTCACAGG | NM_016610 |
| CXCL8 (IL-8) | CTCTTGGCAGCCTTCCTGATTTCT | CAAGGAAAACTGGGTGCAGAGGGT | NM_000584 |