Table 2 Effect of β3-Arg261... | American Society of Hematology

Table 2

Effect of β3-Arg261 substitutions on surface expression, ligand binding, and outside-in signaling–related functions in αIIbβ3 and αvβ3

β3 substitution	Surface expression	Soluble fibrinogen binding	Outside-in signaling–related functions*		Stability in EDTA
β3 substitution	Surface expression	Soluble fibrinogen binding	Adhesion to immobilized fibrinogen and spreading	Clot retraction	Stability in EDTA
αIIbβ3
Arg261Ala	Negligible	Negligible	ND	ND	ND
Arg261Lys	Normal	Normal	Impaired	Impaired	Reduced
αvβ3
Arg261Ala	Normal	Normal	Normal	Normal	Normal
Arg261Lys	Normal	Normal	Normal	Normal	Normal

β3 substitution	Surface expression	Soluble fibrinogen binding	Outside-in signaling–related functions*		Stability in EDTA
β3 substitution	Surface expression	Soluble fibrinogen binding	Adhesion to immobilized fibrinogen and spreading	Clot retraction	Stability in EDTA
αIIbβ3
Arg261Ala	Negligible	Negligible	ND	ND	ND
Arg261Lys	Normal	Normal	Impaired	Impaired	Reduced
αvβ3
Arg261Ala	Normal	Normal	Normal	Normal	Normal
Arg261Lys	Normal	Normal	Normal	Normal	Normal

ND indicates not done.

Outside-in signaling–related functions were examined only when there was adequate surface expression.