Details of the PCR reactions
| Molecule . | Primer . | PCR conditions . |
|---|---|---|
| CD20 | 5′-TGAATGGGCTCTTCCACATTGCC-3′ (forward); 5′-CCTGGAAGAAGGCAAAGATCAGC-3′ (reverse) | 95°C denaturation for 2 minutes, 28 cycles of denaturation at 94°C for 30 seconds, annealing at 54°C for 40 seconds and extension at 72°C for 40 seconds, elongation at 72°C for 10 minutes |
| CD46 | 5′-TGCACTTCTTCCACTACAAAATCTCC-3′ (forward); 5′-ATCCAAACTGTCAAGTATTCCTTCCTC-3′ (reverse) | 95°C denaturation for 2 minutes, 30 cycles of denaturation at 94°C for 30 seconds, annealing at 57°C for 40 seconds and extension at 72°C for 40 seconds, elongation at 72°C for 10 minutes |
| CD55 | 5′-CGTTGCCAGAGTGCAGAGAAA-3′ (forward); 5′-CGTTACAGACTGTCTATATCCATAATC-3′ (reverse) | 95°C denaturation for 2 minutes, 34 cycles of denaturation at 94°C for 30 seconds, annealing at 55°C for 40 seconds and extension at 72°C for 40 seconds, elongation at 72°C for 10 minutes |
| CD59 | 5′-ATGGGAATCCAAGGAGGGT-3′ (forward); 5′-ATGAAGGCTCCAGGCTGCT-3′ (reverse) | 95°C denaturation for 2 minutes, 36 cycles of denaturation at 94°C for 30s, annealing at 52°C for 40s and extension at 72°C for 40s, elongation at 72°C for 10 minutes |
| XBP1 | 5′-CCTTGTAGTTGAGAACCAGG-3′ (forward); 5′-GGGGCTTGGTATATATGTGG-3′ (reverse) | 94°C denaturation for 2 minutes, 36 cycles of denaturation at 94°C for 30 seconds, annealing at 55°C for 30 seconds and extension at 72°C for 30 seconds, elongation at 72°C for 10 minutes |
| actin | 5′-TTCCTTCCTGGGCATGGAGT-3′ (forward); 5′-ATCCACATCTGCTGGAAGGT-3′ (reverse) | 95°C denaturation for 2 minutes, 26 cycles of denaturation at 94°C for 30 seconds, annealing at 55°C for 30 seconds and extension at 72°C for 45 seconds, elongation at 72°C for 10 minutes |
| Molecule . | Primer . | PCR conditions . |
|---|---|---|
| CD20 | 5′-TGAATGGGCTCTTCCACATTGCC-3′ (forward); 5′-CCTGGAAGAAGGCAAAGATCAGC-3′ (reverse) | 95°C denaturation for 2 minutes, 28 cycles of denaturation at 94°C for 30 seconds, annealing at 54°C for 40 seconds and extension at 72°C for 40 seconds, elongation at 72°C for 10 minutes |
| CD46 | 5′-TGCACTTCTTCCACTACAAAATCTCC-3′ (forward); 5′-ATCCAAACTGTCAAGTATTCCTTCCTC-3′ (reverse) | 95°C denaturation for 2 minutes, 30 cycles of denaturation at 94°C for 30 seconds, annealing at 57°C for 40 seconds and extension at 72°C for 40 seconds, elongation at 72°C for 10 minutes |
| CD55 | 5′-CGTTGCCAGAGTGCAGAGAAA-3′ (forward); 5′-CGTTACAGACTGTCTATATCCATAATC-3′ (reverse) | 95°C denaturation for 2 minutes, 34 cycles of denaturation at 94°C for 30 seconds, annealing at 55°C for 40 seconds and extension at 72°C for 40 seconds, elongation at 72°C for 10 minutes |
| CD59 | 5′-ATGGGAATCCAAGGAGGGT-3′ (forward); 5′-ATGAAGGCTCCAGGCTGCT-3′ (reverse) | 95°C denaturation for 2 minutes, 36 cycles of denaturation at 94°C for 30s, annealing at 52°C for 40s and extension at 72°C for 40s, elongation at 72°C for 10 minutes |
| XBP1 | 5′-CCTTGTAGTTGAGAACCAGG-3′ (forward); 5′-GGGGCTTGGTATATATGTGG-3′ (reverse) | 94°C denaturation for 2 minutes, 36 cycles of denaturation at 94°C for 30 seconds, annealing at 55°C for 30 seconds and extension at 72°C for 30 seconds, elongation at 72°C for 10 minutes |
| actin | 5′-TTCCTTCCTGGGCATGGAGT-3′ (forward); 5′-ATCCACATCTGCTGGAAGGT-3′ (reverse) | 95°C denaturation for 2 minutes, 26 cycles of denaturation at 94°C for 30 seconds, annealing at 55°C for 30 seconds and extension at 72°C for 45 seconds, elongation at 72°C for 10 minutes |
PCR indicates polymerase chain reaction.