Receptor expression in ESMCs measured by real time polymerase chain reaction
| Gene tested . | Differentiation by OP9 coculture . | Differentiation by EB formation . | CBMCs . |
|---|---|---|---|
| FCERIA | − | + | ++ |
| MS4A2 | ++ | ++ | ++ |
| FCERIG | +++ | +++ | +++ |
| PTGER1 | − | − | − |
| PTGER2 | + | ++ | + |
| PTGER3 | ++ | ++ | +++ |
| PTGER4 | + | + | + |
| Gene tested . | Differentiation by OP9 coculture . | Differentiation by EB formation . | CBMCs . |
|---|---|---|---|
| FCERIA | − | + | ++ |
| MS4A2 | ++ | ++ | ++ |
| FCERIG | +++ | +++ | +++ |
| PTGER1 | − | − | − |
| PTGER2 | + | ++ | + |
| PTGER3 | ++ | ++ | +++ |
| PTGER4 | + | + | + |
Expression pattern, relative to HPRT gene, of several receptors in mast cells derived by coculture with OP9 cells and from embryoid bodies (EBs) compared with cord blood mast cells (CBMCs) assayed by real-time polymerase chain reaction: − indicates expression < 0.1; +, expression 0.1-1.0; ++, expression 1.0-10.0; +++, expression > 10.0.