Patient characteristics for 12 AML patients for RT-PCR
| Sample . | Age/sex . | Primary/secondary . | De novo/relapsed . | Cytogenetics . | Flt-3 ITD . | % CD34 . | FAB . | Notes . |
|---|---|---|---|---|---|---|---|---|
| 1 | 53/F | Primary | Relapsed | 46, XX, t(5;15;12;12) | Unknown | 50 | M5 | |
| 2 | 56/M | Secondary | De novo | 45, XY, t(8;9)(q24;q22),-21 | Negative | 52 | NS | Secondary to chronic eosinophilic leukemia |
| 3 | 47/M | Primary | De novo | Normal | Negative | 68 | M5 | |
| 4 | 40/F | Primary | De novo | Normal | Normal | 90 | M5 | |
| 5 | 77/F | Primary | De novo | 46, XX, der(7)t(1;7)(q25;q31.2) | Negative | 87 | M4 | FLT3 TKD positive |
| 6 | 59/M | Primary | De novo | Normal | Positive | 18 | NS | |
| 7 | 64/M | Primary | De novo | Normal | Positive | 3 | M1 | |
| 8 | 39/M | Primary | Relapsed | Normal | Positive | 27 | M4 | NPM positive |
| 9 | 61/F | Primary | De novo | 46, XX, t(10;11)(p11.2–12;q23) | Negative | 8 | M5 | MLL positive |
| 10 | 39/F | Primary | De novo | Normal | Positive | 1 | M4 | NPM positive |
| 11 | 57/F | Primary | De novo | Normal | Negative | 4 | M5 | NPM positive |
| 12 | 46/M | Primary | De novo | Failed to grow | Negative | 98 | M5 | FISH negative for monosomy 5,7 and trisomy 8 |
| Sample . | Age/sex . | Primary/secondary . | De novo/relapsed . | Cytogenetics . | Flt-3 ITD . | % CD34 . | FAB . | Notes . |
|---|---|---|---|---|---|---|---|---|
| 1 | 53/F | Primary | Relapsed | 46, XX, t(5;15;12;12) | Unknown | 50 | M5 | |
| 2 | 56/M | Secondary | De novo | 45, XY, t(8;9)(q24;q22),-21 | Negative | 52 | NS | Secondary to chronic eosinophilic leukemia |
| 3 | 47/M | Primary | De novo | Normal | Negative | 68 | M5 | |
| 4 | 40/F | Primary | De novo | Normal | Normal | 90 | M5 | |
| 5 | 77/F | Primary | De novo | 46, XX, der(7)t(1;7)(q25;q31.2) | Negative | 87 | M4 | FLT3 TKD positive |
| 6 | 59/M | Primary | De novo | Normal | Positive | 18 | NS | |
| 7 | 64/M | Primary | De novo | Normal | Positive | 3 | M1 | |
| 8 | 39/M | Primary | Relapsed | Normal | Positive | 27 | M4 | NPM positive |
| 9 | 61/F | Primary | De novo | 46, XX, t(10;11)(p11.2–12;q23) | Negative | 8 | M5 | MLL positive |
| 10 | 39/F | Primary | De novo | Normal | Positive | 1 | M4 | NPM positive |
| 11 | 57/F | Primary | De novo | Normal | Negative | 4 | M5 | NPM positive |
| 12 | 46/M | Primary | De novo | Failed to grow | Negative | 98 | M5 | FISH negative for monosomy 5,7 and trisomy 8 |
Twelve patients were screened by RT-PCR for NuSAP1 and CHAF1b gene expression analysis. The patient characteristics are described.
RT-PCR indicates reverse transcription–polymerase chain reaction; TKD, tyrosine kinase domain; NPM, nucleophosmin; FAB, French-American-British; MLL, mixed lineage leukemia; and FISH, fluorescence in situ hybridization.