Platelet glycoprotein expression in wild-type and Cdc42−/− mice
| . | Wild-type, MFI . | Cdc42−/−, MFI . |
|---|---|---|
| Mean FSC | 345 ± 21 | 440 ± 37 |
| GPIb | 389 ± 8 | 306 ± 11 |
| GPV | 260 ± 4 | 212 ± 3 |
| GPIX | 403 ± 13 | 343 ± 2 |
| CD9 | 1443 ± 21 | 1226 ± 10 |
| GPVI | 61 ± 2 | 52 ± 3 |
| α2 | 90 ± 4 | 90 ± 8 |
| β1 | 162 ± 10 | 183 ± 9 |
| αIIbβ3 | 560 ± 63 | 513 ± 38 |
| . | Wild-type, MFI . | Cdc42−/−, MFI . |
|---|---|---|
| Mean FSC | 345 ± 21 | 440 ± 37 |
| GPIb | 389 ± 8 | 306 ± 11 |
| GPV | 260 ± 4 | 212 ± 3 |
| GPIX | 403 ± 13 | 343 ± 2 |
| CD9 | 1443 ± 21 | 1226 ± 10 |
| GPVI | 61 ± 2 | 52 ± 3 |
| α2 | 90 ± 4 | 90 ± 8 |
| β1 | 162 ± 10 | 183 ± 9 |
| αIIbβ3 | 560 ± 63 | 513 ± 38 |
Expression of glycoproteins on the platelet surface was determined by flow cytometry. Diluted whole blood from the indicated mice was incubated with fluorescein isothiocyanate–labeled antibodies at saturating concentrations for 15 minutes at room temperature, and platelets were analyzed directly. Results are expressed as mean fluorescence intensity ± SD for 6 mice per group. Mean platelet size (mean FSC) was determined by FSC characteristics.
MFI indicates mean fluorescence intensity; FSC, forward scatter; and GP, glycoprotein.