Table 1 Biochemical characterization of... | American Society of Hematology

Table 1

Biochemical characterization of recombinant FIXFc and recombinant and plasma-derived FIX

Characteristic		rFIXFc	rFIX	pdFIX
γ-carboxylation
aa 1-23 (K1K2 peptide)	% 6 Gla	97.8	96.9	99.6
	% 5 Gla	2.2	3.1	0.4
	% 4 Gla	0	0	0
aa 24-43 (K3 peptide)	%6 Gla	61.3	63.7	98.9
	% 5 Gla	26.3	30.9	1.1
	% 4 Gla	12.5	5.4	0
Total Gla/mol, peptide map		11.5	11.6	12.0
Total Gla/mol, AAA		11.2 ± 0.7	11.6 ± 0.5	(12)
Propeptide content		None detected	None detected	None detected
β-hydroxylation Asp 64		70%	49%	37%
Sulfation of Tyr 155		4%	5%	(> 90%)
Phosphorylation of Ser 158		< 10%	< 10%	(> 90%)
Ala 148/Thr 148		Thr	Ala	30% Ala/70%Thr
Activated FIX		< 0.013%	0.11% ± 0.0019%	0.21% ± 0.010%
FXIa activation		94.8% ± 2.4%	96.6% ± 1.8%	Not done

Characteristic		rFIXFc	rFIX	pdFIX
γ-carboxylation
aa 1-23 (K1K2 peptide)	% 6 Gla	97.8	96.9	99.6
	% 5 Gla	2.2	3.1	0.4
	% 4 Gla	0	0	0
aa 24-43 (K3 peptide)	%6 Gla	61.3	63.7	98.9
	% 5 Gla	26.3	30.9	1.1
	% 4 Gla	12.5	5.4	0
Total Gla/mol, peptide map		11.5	11.6	12.0
Total Gla/mol, AAA		11.2 ± 0.7	11.6 ± 0.5	(12)
Propeptide content		None detected	None detected	None detected
β-hydroxylation Asp 64		70%	49%	37%
Sulfation of Tyr 155		4%	5%	(> 90%)
Phosphorylation of Ser 158		< 10%	< 10%	(> 90%)
Ala 148/Thr 148		Thr	Ala	30% Ala/70%Thr
Activated FIX		< 0.013%	0.11% ± 0.0019%	0.21% ± 0.010%
FXIa activation		94.8% ± 2.4%	96.6% ± 1.8%	Not done

Posttranslational modifications and other analyses of rFIXFc, rFIX, and pdFIX were assessed in a variety of assays as described in “γ-carboxylation analysis” and “Additional posttranslational modification and other analyses.” Numbers in parentheses taken from published values.⁷

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