MO sequences
| Gene . | Target . | MO sequence . |
|---|---|---|
| npm1a | ATG/5′UTR | TAATGTTATCCTCCATTTTTGCGCG |
| npm1a | ATG/5′UTR 5-bp mismatch | TAATcTTATCgTCgATTTTaGCcCG |
| npm1a | Exon2-intron2 splice donor (2)* | AAGAAACATCACATACCATTCTAAC |
| npm1a | Exon3-intron3 splice donor | TTATGACCAAGTCTACTTACACTTG |
| npm1b | ATG/5′ UTR | GACCCATCTGTTCGAGATCCATGTC |
| npm1b | ATG/5′UTR 5-bp mismatch | GAgCCATgTGTTCGAcATCgATcTC |
| npm1b | Exon2-intron2 splice donor | TCAAATATTCATCTTCCTCACCGAC |
| p53 | ATG/5′UTR | GCGCCATTGCTTTGCAAGAATTG |
| Std cont | Human β-globin intron mutation | CCTCTTACCTCAGTTACAATTTATA |
| Gene . | Target . | MO sequence . |
|---|---|---|
| npm1a | ATG/5′UTR | TAATGTTATCCTCCATTTTTGCGCG |
| npm1a | ATG/5′UTR 5-bp mismatch | TAATcTTATCgTCgATTTTaGCcCG |
| npm1a | Exon2-intron2 splice donor (2)* | AAGAAACATCACATACCATTCTAAC |
| npm1a | Exon3-intron3 splice donor | TTATGACCAAGTCTACTTACACTTG |
| npm1b | ATG/5′ UTR | GACCCATCTGTTCGAGATCCATGTC |
| npm1b | ATG/5′UTR 5-bp mismatch | GAgCCATgTGTTCGAcATCgATcTC |
| npm1b | Exon2-intron2 splice donor | TCAAATATTCATCTTCCTCACCGAC |
| p53 | ATG/5′UTR | GCGCCATTGCTTTGCAAGAATTG |
| Std cont | Human β-globin intron mutation | CCTCTTACCTCAGTTACAATTTATA |
Bold letters indicate the ATG codon; lowercase letters, mismatched residues; and Std cont, standard control.
(2) is the 1 working morpholino of 2 tested (supplemental Figure 1F, available on the Blood Web site; see the Supplemental Materials link at the top of the online article).